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Procurable · sourced on request

Sigma-Aldrich F3165

UsedSigma-AldrichImmunoassay reagents / antibodiesNetherlands
Procurable

Not in stock, available to order through our sourcing team

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Antibody Form
Purified immunoglobulin (IgG1 subclass)
Buffer Composition
10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide
Purification Method
Protein A affinity purification
Isotype
IgG1
Concentration
3.8-4.2 mg/mL
Availability
Procurable
Lead time
Confirmed with your quote
All 14 specifications
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Specifications 14

Brands
Sigma-Aldrich
New/Used
Used
Antibody Form
Purified immunoglobulin (IgG1 subclass)
Buffer Composition
10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide
Purification Method
Protein A affinity purification
Isotype
IgG1
Concentration
3.8-4.2 mg/mL
Immunogen Sequence
DYKDDDDK
Species Reactivity
All species
Storage Temperature
-20°C
Shipping Conditions
Dry ice
Recommended Dilution for Western Blot
10 µg/mL
Availability
Procurable
Lead time
Confirmed with your quote

Product description

Mouse monoclonal antibody targeting FLAG epitope, calcium-independent binding, suitable for multiple immunoassays.

The Monoclonal ANTI-FLAG® M2 Antibody (F3165) is a highly specific mouse-derived monoclonal antibody designed to detect and purify FLAG-tagged fusion proteins. It recognises the FLAG epitope sequence (DYKDDDDK) at the N-terminus, Met N-terminus, C-terminus, and internal sites of target proteins, facilitating versatile applications in protein research.

Key features:

  • High specificity: Targets the FLAG sequence with minimal cross-reactivity.
  • Versatile applications: Suitable for immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, ELISA, chromatin immunoprecipitation, electron microscopy, flow cytometry, and supershift assays.
  • Calcium-independent binding: Unlike M1 antibodies, M2 binding is not calcium-dependent, ensuring consistent performance across various conditions.
  • Purified immunoglobulin: Affinity-purified using Protein A, enhancing sensitivity and reducing background noise.

This antibody is widely utilised in biotechnology, molecular biology, and biomedical research for the detection and purification of FLAG-tagged proteins. Its applications are critical in protein expression studies, protein-protein interaction analyses, and structural biology.

Commonly employed in university laboratories, biomedical research centres, and biopharmaceutical companies, the ANTI-FLAG® M2 Antibody has been a staple in research settings since its introduction. Its enduring relevance underscores its reliability and effectiveness in advancing scientific discoveries.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 23 October 2026