Sigma-Aldrich A5441

Monoclonal Anti-β-Actin antibody (Sigma A5441) is a highly specific mouse-derived antibody targeting the β-actin protein, a crucial component of the cytoskeleton involved in various cellular processes. This antibody is widely utilised as a loading control in western blot analyses, ensuring accurate quantification of target proteins across diverse samples.

Key features include:

• High specificity: Recognises an epitope on the N-terminal end of β-actin, ensuring precise detection.
• Versatile applications: Suitable for western blotting, immunohistochemistry, immunofluorescence, and ELISA techniques.
• Broad species reactivity: Effective across multiple species, including human, mouse, rat, and bovine.
• Consistent performance: Provides reliable results, making it a standard in protein expression studies.

This antibody is extensively applied in:

• Molecular biology: Assessing protein expression levels in various cell types.
• Biotechnology: Validating protein production in recombinant systems.
• Clinical diagnostics: Serving as a control in diagnostic assays.
• Pharmaceutical research: Evaluating drug effects on protein expression.

Commonly utilised in:

• University laboratories: For fundamental research and teaching purposes.
• Biotechnology companies: In product development and quality control.
• Clinical research facilities: For diagnostic assay development and validation.
• Pharmaceutical firms: In drug discovery and development pipelines.

First introduced in the late 20th century, this antibody remains a cornerstone in protein analysis, reflecting its enduring relevance in scientific research.