Sigma-Aldrich A1978

Anti-β-Actin Antibody (Product No. A1978) is a monoclonal antibody designed to detect β-actin, a fundamental component of the cytoskeleton involved in various cellular processes. This antibody is widely used as a loading control in Western blotting and other immunodetection techniques, ensuring accurate protein quantification across samples.

Key features include:

• High specificity: Recognises an epitope on the N-terminal end of the β-actin isoform, ensuring precise detection.
• Versatile applications: Suitable for Western blotting, immunocytochemistry, immunohistochemistry (on frozen sections), indirect immunofluorescence, and microarray analyses.
• Broad species reactivity: Effective across multiple species, including human, mouse, rat, bovine, and chicken.
• Robust performance: Epitope is resistant to formalin-fixation and paraffin-embedding, facilitating use in various sample preparations.

This antibody is extensively utilised in:

• Molecular biology: For studying protein expression and cellular dynamics.
• Cell biology: To investigate cytoskeletal structures and functions.
• Biotechnology: As a control in protein assays and diagnostic applications.

Commonly employed in:

• University laboratories: For academic research and teaching purposes.
• Biomedical research centres: In studies related to cell structure and function.
• Biotechnology companies: For product development and quality control processes.

First introduced in the late 20th century, this antibody remains a staple in research laboratories due to its reliability and versatility.