Cell Signaling Technology 7074

Anti-rabbit IgG, HRP-linked Antibody (Cat. #7074) is an affinity-purified goat anti-rabbit IgG antibody conjugated to horseradish peroxidase (HRP), designed for the detection of rabbit primary antibodies in various immunoassays.

• Key Features:
• High specificity for rabbit IgG (heavy and light chains), ensuring minimal cross-reactivity.
• HRP conjugation enables sensitive chemiluminescent detection.
• Validated for use with Cell Signaling Technology (CST) primary antibodies, ensuring optimal performance.
• Recommended dilutions: 1:1,000–1:3,000 for Western blotting applications.
• Stable formulation: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA), and 50% glycerol; stable for 60 months when stored at –20°C.

• Applications:
• Western blotting: Facilitates the detection of target proteins in complex samples.
• Immunoprecipitation: Assists in the identification and analysis of protein complexes.
• Immunohistochemistry: Enables visualization of antigen distribution in tissue sections.
• ELISA: Supports quantitative measurement of antigens in various samples.

• Research Domains:
• Molecular biology
• Cell biology
• Biotechnology
• Immunology
• Neuroscience

• Commonly Used In:
• University laboratories
• Biomedical research centres
• Clinical research facilities
• Biotechnology companies

First released prior to 2015, this antibody remains a widely adopted tool in the scientific community, particularly in biotechnology and molecular biology research. Its high specificity and sensitivity make it essential for accurate protein detection, contributing to advancements in areas such as gene expression analysis, signal transduction studies, and biopharmaceutical development.