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BD Biosciences LSRII

UsedBD BiosciencesFlow cytometersNetherlands
Procurable

Not in stock, available to order through our sourcing team

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Acquisition Rate
>20000 events per second
Fluorescence Sensitivity
<200 molecules of equivalent soluble fluorochrome (MESF)
Fluorescence Resolution
<3% Coefficient of Variation (CV) for FL-Area
Fluorescence Linearity
Doublet/singlet ratio for CENs stained with PI or Hoechst is 1.95-2.05
Forward and Side Scatter Sensitivity
Sensitivity enables the separation of fixed platelets from noise
Availability
Procurable
Lead time
Confirmed with your quote
All 17 specifications
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Specifications 17

Brands
BD Biosciences
New/Used
Used
Acquisition Rate
>20000 events per second
Fluorescence Sensitivity
<200 molecules of equivalent soluble fluorochrome (MESF)
Fluorescence Resolution
<3% Coefficient of Variation (CV) for FL-Area
Fluorescence Linearity
Doublet/singlet ratio for CENs stained with PI or Hoechst is 1.95-2.05
Forward and Side Scatter Sensitivity
Sensitivity enables the separation of fixed platelets from noise
Digital Signal Processing
Digital electronics with 10 MHz sampling frequency and 18-bit data acquisition for area signals
Laser Wavelengths and Powers
355 nm (20 mW), 405 nm (25 mW), 488 nm (20 mW), 640 nm (40 mW)
Sample Flow Rates
LO = 12 µL/min, MED = 35 µL/min, HI = 60 µL/min
Fluidic Reservoirs
One 8-L sheath and 10-L waste container provided
Software
BD FACSDiva™ Software Version 6.1
Detection Limits
<200 molecules of equivalent soluble fluorochrome (MESF)
Measurement Range
Capable of detecting up to 18 colours simultaneously
Connectivity/Interface
BD FACSDiva™ Software for data acquisition and analysis
Availability
Procurable
Lead time
Confirmed with your quote

Product description

High-performance flow cytometer with up to 18-color detection and 4 lasers for advanced cellular analysis.

BD Biosciences LSRII is a high-performance flow cytometer designed for advanced multicolour analysis in biomedical research. Introduced in 2004, this instrument has been instrumental in facilitating complex cellular analyses across various scientific disciplines.

Key features include:

  • Multicolour Detection: Capable of detecting up to 18 colours simultaneously, enabling comprehensive phenotypic profiling.
  • Multiple Laser Options: Equipped with up to four lasers (355 nm UV, 405 nm, 488 nm, and 633 nm), offering flexibility in fluorochrome selection.
  • Digital Signal Processing: Utilises digital electronics that sample signals at 10 MHz with 18-bit resolution, enhancing sensitivity and data accuracy.
  • High Acquisition Speed: Processes over 20,000 events per second, suitable for high-throughput applications.
  • Advanced Software: Integrated with BD FACSDiva™ software for efficient data acquisition and analysis.

The LSRII has been widely adopted in fields such as immunology, cancer research, neuroscience, and biotechnology. Its capabilities are essential for applications like cellular phenotyping, cytokine profiling, and cell cycle analysis.

Commonly utilised in university laboratories, biomedical research centres, and clinical research facilities, the LSRII has been a cornerstone in advancing flow cytometry applications.

Please note that BD Biosciences discontinued the sale of the LSRII in 2017, with service support concluding in 2022. Researchers seeking similar capabilities are encouraged to explore BD's current range of research cell analysers for advanced flow cytometry analyses.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 22 October 2026