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Beckman Coulter FC 500

UsedBeckman CoulterFlow cytometersNetherlands
Procurable

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Dimensions (L x W x H)
889 x 991 x 610 mm
Laser Excitation Wavelengths
488 nm, 633 nm
Laser Output Power
20 mW
Particle Size Detection Range
0.5 to 40 µm
Fluorescence Sensitivity (FITC)
< 600 MESF
Availability
Procurable
Lead time
Confirmed with your quote
All 31 specifications
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Specifications 31

Brands
Beckman Coulter
New/Used
Used
Dimensions (L x W x H)
889 x 991 x 610 mm
Weight
85 kg
Laser Excitation Wavelengths
488 nm, 633 nm
Laser Output Power
20 mW
Particle Size Detection Range
0.5 to 40 µm
Fluorescence Sensitivity (FITC)
< 600 MESF
Fluorescence Sensitivity (PE)
< 300 MESF
Fluorescence Sensitivity (APC)
< 600 MESF
Maximum Acquisition Rate
3,300 events/sec
Sample Tube Size
12 x 75 mm
Sample Tube Capacity
32 tubes
Flow Rates
Low: ~15 µL/min, Medium: ~30 µL/min, High: ~60 µL/min
Flow Cell Dimensions
150 x 450 µm
Flow Cell Channel Type
Rectangular
Flow Cell Material
Quartz
Flow Cell Flow Path
Vertical (upwards)
Flow Cell Optics
BioSense enhanced optics
Flow Cell Mounting
Quartz mounted
Flow Cell Hydrodynamic Focusing
Superior
Flow Cell Design
BioSense enhanced optics quartz mounted with vertical (upwards) flow path for superior hydrodynamic focusing
Operating Temperature Range
16°C to 32°C
Power Supply
110V or 220V
Power Consumption
120 lbs (54.4 kg)
Computer Dimensions (L x W x H)
406 x 533 x 432 mm
Computer Weight
18.14 kg
Monitor Dimensions (L x W x H)
432 x 406 x 444 mm
Monitor Weight
22 kg
Availability
Procurable
Lead time
Confirmed with your quote

Product description

Benchtop flow cytometer with dual-laser excitation, five-color analysis, and automated sample handling.

The Beckman Coulter FC 500 is a sophisticated benchtop flow cytometer designed to deliver flexible testing capabilities, powerful software, and a user-friendly interface, enhancing workflow efficiency and cost-effectiveness in laboratory settings.

Key features include:

  • Dual-laser excitation: Equipped with a 488 nm air-cooled argon ion laser and a 633 nm helium-neon laser, enabling simultaneous five-colour analysis for comprehensive cellular profiling.
  • High sensitivity and resolution: Capable of detecting particles ranging from 0.5 µm to 40 µm in diameter, with fluorescence sensitivity of <600 MESF for FITC and APC, and <300 MESF for PE, ensuring precise detection of low-abundance populations.
  • Flexible sample handling: Accommodates 12 x 75 mm tubes and offers options for single-tube sampling or automated processing of up to 32 tubes via a multi-carousel loader, catering to varying throughput requirements.
  • Advanced digital signal processing: Maintains stable alignment with a single beam path, providing excellent, drift-free linearity and the flexibility to recompensate after acquisition, enhancing data accuracy.
  • Comprehensive reagent support: Access to over 1,000 reagents, including customisable options with various fluorochromes, facilitating tailored assay development for diverse research needs.

This instrument is widely utilised in biotechnology, immunology, haematology, and clinical diagnostics for applications such as cellular immunophenotyping, leukaemia and lymphoma diagnostics, and immune function analysis. It is commonly employed in university laboratories, biomedical research centres, and clinical diagnostic facilities. Introduced in 2002, the FC 500 has been a reliable tool in flow cytometry; however, users should be aware of a Class 1 recall issued in 2018 due to potential circuit amplifier board failures, which could impact assay results.

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Frequently asked questions

How long does laboratory equipment typically last?

With a service contract, ten to fifteen years is normal for most benchtop instruments. Detectors and pumps are the parts that wear first.

Can I get service for older equipment?

Often, yes. Verified suppliers frequently work with service partners converting the instrument. If applicable, we can list servicing options alongside the offer.

Where can I buy parts?

Ask a scientist through the request button. Parts are often available even when the instrument itself is discontinued.

What is the typical lead time for refurbished equipment?

Two to six weeks, depending on refurbishment scope and whether calibration is included.

Does laboratory equipment ship internationally?

We ship to 38 countries. Crating and customs handling are quoted with the instrument.

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Last updated 4 November 2026