Sartorius Biostat B Benchtop Twin Bioreactor System for Single-Use Univessel Cell Culture

The Sartorius Biostat B Benchtop Bioreactor SU Univessel System, Twin is a dual-vessel bioprocess control platform specifically designed for small-scale mammalian cell culture and biotechnology workflows involving single-use stirred tank bioreactors. This system enables parallel cultivation with independent control of two 0.6–2 L working volume SU Univessels, facilitating bioprocess development, seed train expansion, and scalable cell culture experiments critical in molecular diagnostics and biopharmaceutical production pipelines.

Key technical features include:

• Dual stainless steel control towers managing two single-use Univessel SU holders equipped with Sensolux illumination
• Integrated 4-gas mixing system (Air, O₂, N₂, CO₂) controlled via Thermal Mass Flow Controllers (TMFCs) with ±1% full-scale precision for accurate gas supply and sparging essential for optimizing cell respiration and metabolic activities in suspension cultures
• Independent direct drive agitation motors (200 W each) supporting 20–2000 rpm speed range, suitable for controlled mixing and shear-sensitive cell culture conditions typical in synthetic biology and gene editing research
• External Watson-Marlow 120U peristaltic pumps for precise feed, base addition, and antifoam dosing, incorporating variable speed control (0–200 rpm) for optimized media delivery and bioprocess feeding strategies
• Temperature regulation using external heating blankets with up to 50 °C control range and 220 W heating capacity, ensuring stable culture environment critical for protein expression and viable cell growth
• Extensive digital interfacing: integrated sensors for pH/redox, optical dissolved oxygen (DO), inline biomass monitoring (BioPAT ViaMass), vessel weight scales with RS-232 communication, and analog inputs (0–10 V / 4–20 mA) for enhanced process monitoring and control
• Dimensions: 35×82.2×43 cm; Weight: 55 kg; Power: 120 V, 60 Hz, max 12 A, supporting standard laboratory electrical infrastructure

This bioreactor system is widely adopted in biotechnology fields for live-cell imaging studies, fluorescence-based monitoring, and as an essential tool in molecular cloning workflows due to its sterile single-use design and flexibility to accommodate both disposable and autoclavable vessels upon inquiry. It supports parallel bioprocess optimization integral for early-stage drug development, biomanufacturing scale-up, and clinical diagnostics research.

Consumables such as single-use Univessel SU vessels are not included. Optional customizations and sensors, including pH and oxygen probes, stirrer options, vessel scales, and exhaust analyzers, are available to tailor the system for specific applications in bioprocess engineering and cell line development.

The system is supplied fully serviced with remote setup, technical consultation, and a warranty covering 120 days within the continental US and 60 days internationally. The instrument undergoes rigorous functional verification including touchscreen operation, pump performance across RPM ranges, temperature sensor accuracy, agitation motor validation, and gas mixing calibration using traceable standards.

This benchtop bioreactor controller facilitates efficient parallel mammalian cell culture and bioprocess development with flexibility and precision, making it a critical asset for research institutions, biopharmaceutical companies, and universities involved in cutting-edge synthetic biology and molecular diagnostics.