Cytiva Sephacryl S-200 HR

Sephacryl® S-200 High Resolution is a gel filtration chromatography medium designed for the purification of antibodies, serum proteins, and mid-sized proteins. This medium is widely used in biotechnology and molecular biology laboratories for its efficiency in size exclusion chromatography.

Key features include:

• Fractionation range: Effectively separates globular proteins within a molecular weight range of 5,000 to 250,000 Daltons.
• Matrix composition: Constructed from a cross-linked copolymer of allyl dextran and N,N′-methylene bisacrylamide, providing rigidity and chemical stability.
• Particle size: Narrow distribution with an average size of 50 μm, ensuring high-resolution separations.
• Chemical stability: Stable across a pH range of 3 to 11 and compatible with various solvents, including 1M acetic acid, 8M urea, and 6M guanidine-HCl.
• Autoclavable: Can be sterilised at 121°C, pH 7 for 30 minutes, facilitating reuse and maintaining sterility.

This medium is extensively applied in biotechnology, molecular biology, and biopharmaceutical research for:

• Purification of antibodies and serum proteins.
• Isolation of mid-sized proteins for structural and functional studies.
• Development of high-throughput screening assays, such as those for sickle cell disease drug discovery.

Commonly utilised in university laboratories, biomedical research centres, and biopharmaceutical companies, this medium supports both laboratory-scale and industrial-scale applications. Its long-term physical and chemical stability, combined with ease of handling, makes it a preferred choice for routine purification tasks.

First introduced prior to 2025, Sephacryl® S-200 High Resolution continues to be a relevant and reliable tool in the field of protein purification.