Eksigent NanoLC-2D, 920 Two-Dimensional Automated Nano HPLC System for Peptide Separation

The Eksigent NanoLC-2D, 920 is a precision two-dimensional high-performance liquid chromatography (2D HPLC) system engineered for advanced peptide separation in LC/MS proteomics workflows. Utilizing an independent microfluidic direct pumping system, it delivers:

• First dimension flow rates ranging from 1 to 20 µL/min
• Second dimension nano-flow between 20 to 1000 nL/min
• High-pressure gradient mixing eliminates flow splitting, enhancing gradient accuracy
• Ultra-low delay volumes of 300 nL (first dimension) and 65 nL (second dimension) for minimal band broadening
• Supports multiple injection modes: standard, metered, and rapid, with injection volumes from 50 to 200 µL and options for larger sample input
• Compatible with mobile phases contacting components made from 316 stainless steel, PEEK, and silica, suitable for diverse chromatographic chemistries
• Optimized columns include 300-500 µm i.d. traps or columns (5 cm length) for the first dimension and 50-150 µm i.d. columns (5-15 cm length) for the second dimension, facilitating robust peptide fractionation protocols such as MudPIT

This system maintains a retention time relative standard deviation (RSD) of less than 0.5% at 500 nL/min, ensuring reproducible separation critical for sensitive molecular diagnostics and bioprocessing applications. Its robust design and automated control make it highly valuable for proteomics, molecular biology, and synthetic biology research requiring precise, reproducible two-dimensional peptide separations. The Eksigent NanoLC-2D, 920 is essential equipment for laboratories specializing in biotechnology research, molecular cloning workflows, and biopharmaceutical production pipelines, supporting advanced peptide fractionation and LC/MS integration for biomarker discovery and peptide mapping.