Supelco Supelcosil LC-8-DB (58982) Reversed-Phase HPLC Column 7.5 cm × 4.6 mm × 3 µm

The Supelco Supelcosil LC-8-DB (58982) is a high-performance reversed-phase liquid chromatography column featuring dimensions of 7.5 cm length, 4.6 mm internal diameter, and 3 µm particle size. It employs a C8 stationary phase that is less hydrophobic than traditional C18 phases, resulting in reduced retention for both polar and non-polar analytes compared to C18 columns. This column is designed for optimized separation in analytical workflows requiring precise control of retention times, particularly in biotechnology applications such as molecular diagnostics, bioprocessing, and gene editing workflows.

Key technical features include:

• C8 phase chemistry providing moderately hydrophobic interactions, enabling selective retention of analytes with intermediate polarity.
• Reduced retention of analytes compared to C18, facilitating faster elution with less organic modifier—typically, a mobile phase with about 5% less organic solvent is required relative to C18 protocols.
• Enhanced relative retention of polar compounds, aiding their resolution in complex mixtures.
• Suitable for biomedical engineering, synthetic biology, molecular cloning, and biopharmaceutical production pipelines requiring reliable chromatographic separation.

This column is ideal for researchers and analysts aiming for reproducible reversed-phase separations with moderate hydrophobicity, supporting high-throughput chromatography in clinical diagnostics, cell imaging assays, and quality control in biotechnology manufacturing.