Roche LightCycler 480 II 96-Well Real-Time PCR System with Integrated UPS and Computer

The Roche LightCycler 480 II is an advanced real-time PCR system designed for high-throughput molecular diagnostics and biotechnology applications, including gene expression profiling, genotyping, and mutation scanning critical in bioprocessing and synthetic biology research. This 96-well plate-based thermal cycler uses an improved Peltier-based silver thermal block enabling exceptional well-to-well temperature uniformity and reproducibility, essential for precise nucleic acid quantification.

Key features include:

• Reaction Volume: Flexible 10 μL–100 μL per well, suitable for varied experimental requirements.
• Temperature Range: Programmable from 37°C to 95°C with precise control enabling specific melting curve analyses required for mutation detection assays.
• Optical System: Uniform signal collection across the entire plate, independent of sample position, supporting multiplex fluorescence detection via multiple probe chemistries such as SYBR Green I, ResoLight dye for high-resolution melting, hydrolysis probes, HybProbe, and SimpleProbe.
• Software: Pre-installed LightCycler 480 Software allows intuitive real-time PCR programming, automated data capture, and complex data analysis workflows, including automated evaluation via macro scripting—critical for scalable gene expression studies and large-scale molecular cloning workflows.
• Applications: Widely adopted in biotechnology, clinical diagnostics, biomedical engineering, and synthetic biology, the system ensures reliable high-throughput quantitative and qualitative nucleic acid analyses, crucial for molecular diagnostics and biomanufacturing pipelines.

Included is a Toshiba 1000 Series 3.0 kVA UPS (Uninterruptible Power Supply), providing robust power protection for uninterrupted operation in sensitive laboratory environments. This true online double-conversion UPS features:

• 230V single-phase input compatibility with wide input voltage and frequency tolerance for generator use.
• Auto-restart, cold start capability, rapid battery recharge (to 90% in 4 hours), and programmable load shed receptacles.
• Battery configuration requires six 12V 9Ah batteries.

Also included is an HP Engage Flex Pro computer with preloaded LightCycler software, LCD monitor, keyboard, and wired mouse for seamless system control and data management.

This integrated system is optimal for research professionals requiring a reliable, precise, and versatile real-time PCR platform, essential for advanced molecular biology, gene editing projects, and high-throughput biopharmaceutical production.