Roche Diagnostics LightCycler 96 Real-Time PCR System Installation Package

The Roche Diagnostics LightCycler 96 is a 96-well real-time PCR and quantitative PCR system designed for rapid amplification, fluorescence detection, and molecular analysis. Its Peltier temperature-control system supports 3-step, 40-cycle PCR runs in 40 minutes or less, depending on the enzyme.

• Sample capacity: 96 samples per run
• Reaction formats: 0.1 mL block, 8-strip tubes, and 96-well plates
• Reaction volume: 10–50 µL per well
• Temperature range: 37–98 °C
• Heating rate: 4.4 °C/s
• Cooling rate: 2.2 °C/s
• Temperature accuracy: ±0.2 °C
• Temperature uniformity: ±0.4 °C
• Gradient range: 37–98 °C, with a maximum gradient setting of 20 °C
• Fluorescence excitation: High-power broad-spectrum LED with 470, 533, 577, and 645 nm wavelengths
• Fluorescence detection: 514, 572, 620, and 697 nm wavelengths
• Detection configuration: Four fixed excitation/detection combinations with 4-color multiplexing
• Detector: Glass-fiber excitation and detection system with CCD camera detection
• Supported dyes: SYBR Green I, FAM, ResoLight, VIC, HEX, Yellow555, Red610, Texas Red, and Cy5
• Assay formats: Intercalating dyes and hydrolysis probes
• Analysis functions: Absolute quantification, relative quantification, Tm calling, endpoint genotyping, HRM, and qualitative detection
• Operation and connectivity: Integrated touchscreen control, USB memory support, and LAN connectivity; PC-free operation is supported
• Dimensions: 400 × 400 × 530 mm (W × D × H)
• Weight: Approximately 27 kg
• Power: AC 100–125 V ±10%, 600 W, 50/60 Hz
• Operating noise: 43 dB or less

This used real-time PCR instrument is suitable for molecular biology, biotechnology, gene expression analysis, genotyping, high-resolution melt analysis, and molecular diagnostics workflows.