Shimadzu Nexera Hybrid UHPLC System with Dual LC-20ADXR Pumps, SIL-30ACMP Autosampler and SPD-40 UV-Vis Detector

The Shimadzu Nexera Hybrid UHPLC system is a configured binary-gradient liquid chromatography platform for pharmaceutical analysis, environmental testing, food safety, clinical and bioanalytical studies, chemical purity testing, and academic method development. The system combines dual LC-20ADXR pumps, an SPD-40 UV-Vis detector, SIL-30ACMP autosampler, CTO-20A column oven, DGU-20A5R degassing unit, and CBM-20A system controller.

System Configuration

• LC-20ADXR pumps
• Two pumps for binary high-pressure/low-pressure mixing gradients
• Flow rate range: 0.0001–10 mL/min
• Flow rate precision: no more than 0.06% RSD or 0.02 min SD, whichever is greater
• Gradient/concentration precision: ±0.5% under specified conditions
• Maximum pressure: 66 MPa at 0.0001–3 mL/min
• Maximum pressure: 44 MPa at 3.0001–5 mL/min
• Pump serial numbers: L20435352920 and L20435352918
• The front-panel PUMP and PURGE buttons are inactive; pump control is performed through the software

• DGU-20A5R degassing unit
• 5 degassing flow lines
• Up to 10 mL/min per line
• UV difference: 300 mAU at 210 nm using methanol at 1 mL/min
• Degassing efficiency: approximately 89% at 1 mL/min and 25% at 10 mL/min
• Internal volume: 400 µL per line
• Pressure tolerance: ±0.1 MPa, suction only

• SPD-40 UV-Vis detector
• Deuterium (D2) lamp
• Wavelength range: 190–700 nm
• Bandwidth: 8 nm
• Wavelength accuracy: ±1 nm
• Wavelength reproducibility: ±0.1 nm
• Drift: ≤0.1 × 10−3 AU/h under specified conditions
• Noise in 1-wavelength mode: ≤4.0 × 10−6 AU
• Noise in 2-wavelength mode: ≤10.0 × 10−6 AU
• Linearity: 2.5 AU
• Conventional analytical flow cell, part number 228-68250-42
• Optical path length: 10 mm
• Flow cell volume: 12 µL
• Cell temperature control: 19–50°C in 1°C steps
• The D2 lamp has 9 hours of use compared with a stated 2000-hour lifetime

• CTO-20A column oven
• Forced-air circulation
• Temperature control range: ambient +10°C to 85°C
• Temperature setting range: 4–85°C in 1°C steps
• Temperature control precision: ±0.1°C
• Ambient temperature range: 4–35°C

• SIL-30ACMP autosampler
• Injection volume up to 50 µL
• Supports 6 microtiter plates, including 96-well, 384-well, and deep-well plates, or 1.5 mL vial racks
• Configured with DWP96 deep-well plate racks
• Capacity of up to 576 samples with 96-well plates
• Capacity of up to 2304 samples with 384-well plates
• Capacity of up to 324 vial positions
• Integrated rinse system with dedicated rinse lines to reduce carryover
• New needle installed
• High- and low-pressure valve rotors have been used for less than 1% of their service life

Included Components

• CBM-20A system controller
• Solvent reservoir tray
• POWERVAR power conditioner, part number 61100-59R
• Computer with LabSolutions software and dongle
• Communication and power cables
• Conventional analytical flow cell
• System manuals for the LC-20ADXR pump, SIL-30ACMP autosampler, CBM-20A controller, DGU-20A5R degasser, and CTO-20A column oven

Testing and Condition

The pre-owned UHPLC system was powered on, initialized, and tested by the in-house service team. Testing included module communication, software communication, pump flow stability and accuracy, pressure pulsation, leak sensors, pressure limiters, degasser vacuum performance, detector wavelength accuracy, baseline noise and drift, autosampler purging, cooling, injection repeatability, injection accuracy, and column oven temperature stability.

The DGU-20A5R degasser reached a vacuum level of −95 kPa. The system is described as fully functional and in good cosmetic condition.

This Shimadzu Nexera Hybrid configuration is suitable for UHPLC method development, pharmaceutical quality control, impurity profiling, environmental contaminant analysis, clinical bioanalysis, and biotechnology workflows requiring automated sample handling and UV-Vis absorbance detection.