Thermo Scientific Vanquish Flex Quaternary UHPLC System with Variable Wavelength Detector

The Thermo Scientific Vanquish Flex Quaternary UHPLC System is a modular high-performance liquid chromatography platform configured for pharmaceutical analysis, impurity profiling, environmental testing, food analysis, clinical research, natural products characterization, and HPLC/UHPLC method development. The system combines quaternary solvent delivery, split-loop autosampling, temperature-controlled column separation, post-column cooling, and variable-wavelength UV detection.

Included configuration

• Vanquish Quaternary Pump F, with static mixer, 350 µL volume, p/n VF-P20-A
• Vanquish Split Sampler FT, p/n VF-A10-A
• Vanquish Column Compartment H with post-column cooler, p/n VH-C10-A
• Vanquish Variable Wavelength Detector F, p/n VF-D40-A
• Semi-micro, biocompatible flow cell, 2.5 µL, 7 mm, 5 MPa, Bio p/n 6077.0300
• Solvent rack with bottles
• Intercommunication cables, standard power cords, and accessory kit

Variable Wavelength Detector F

• UV/VIS/NIR photometer with tunable Czerny-Turner monochromator and internal reference beam
• Deuterium lamp for UV and VIS; tungsten lamp for VIS and NIR
• Wavelength range: 190–900 nm
• Spectral bandwidth: typically 6 nm at 254 nm
• Wavelength accuracy: ±1 nm
• Wavelength repeatability: ±0.1 nm
• Internal wavelength calibration using the zero order of the monochromator spectrum
• Internal wavelength validation using a holmium-oxide filter
• Up to 4 signal channels
• Data collection rate: up to 250 Hz in single-channel mode and up to 5 Hz in multi-channel mode
• Noise: <±2.5 µAU at 254 nm in single-channel mode
• Multi-channel noise: <±10 µAU, typically <±7 µAU, at 254 nm and 280 nm
• Drift: <0.1 mAU/h at 254 nm
• Linearity: <5% at 2.5 AU, typically <3% at 2.5 AU

Quaternary Pump F

• Serial dual-piston operating principle
• Flow range: 0.001–8 mL/min, adjustable in 1 µL/min increments
• Pressure range: 2–103 MPa; above 5 mL/min, the pressure range decreases to 80 MPa
• Fully automated compressibility compensation independent of mobile-phase composition
• Flow accuracy: ±0.1%
• Flow precision: <0.05% RSD or <0.01 min SD, whichever is greater
• Typical pulsation: <1.0% or <0.2 MPa, whichever is greater
• Low-pressure gradient proportioning with 4 solvent lines
• Proportioning accuracy: ±0.5% of full scale
• Proportioning precision: <0.15% SD
• Static mixer volume: 350 µL
• Dwell volume: 679 µL
• Built-in solvent degassing for 4 channels

Split Sampler FT

• 1 injection unit using split-loop injection
• Pressure range: 2–103 MPa
• Injection volume range: 0.01–25 µL, with a minimum step of 0.01 µL
• Injection volume accuracy: typically ±0.5% for 10 µL water
• Injection precision: <0.25% area RSD for 1 µL caffeine in water; typically <0.5% area RSD for 0.5 µL
• Injection linearity: r > 0.99999 with caffeine in water
• Injection cycle time: down to 8 seconds, depending on separation conditions
• Minimum sample required: 2 µL at a 1 µL injection volume
• UV carryover: <0.002% with caffeine; typically <0.0004%
• External needle wash with 1 solvent per injection unit, dip rinse, and continuous rinse
• Sample compartment temperature range: 4–40 °C, at least 23 K below ambient at less than 80% RH
• Sample temperature accuracy: −2 °C/+4 °C
• Sample temperature stability: ±1 °C
• Autosampler gradient-delay contribution: 110 µL with a 25 µL sample loop
• Capacity for 4 segments supporting SBS-format sample racks or well plates
• Carousel capacity for 12 × 22.5 mm outer-diameter vials
• Barcode functions include empty-segment detection, rack/well-plate verification, and inventory management

Column Compartment H and post-column cooler

• Column chamber uses still-air and forced-air operation
• Column temperature range: 5–120 °C in 0.1 °C increments, up to 18 K below ambient
• Column temperature stability: ±0.05 K
• Column temperature accuracy: ±0.5 K up to 80 °C
• Heating performance: <15 minutes from 20–50 °C at ±1 K; typically 5 minutes from 25–40 °C at ±1 K
• Cooling performance: <15 minutes from 50–20 °C at ±1 K
• Capacity: 2 column slots
• Passive post-column cooler
• Post-column cooler temperature range: 40–80 °C
• Post-column cooler stability: ±0.05 K
• Post-column cooler accuracy: ±2 K up to 80 °C
• Cooling to 50 °C at 2 mL/min with a 120 °C column temperature

This Thermo Scientific UHPLC system is suited to reproducible liquid chromatography workflows in pharmaceutical quality control, biotechnology, molecular diagnostics, biomedical research, and analytical method validation. Additional detector flow cells, ELSD detectors, mass spectrometer interfaces, and fraction collectors may be available separately.