Dionex BioLC AD25 Dual-Beam Variable Wavelength Absorbance Detector with DX-LAN Interface

The Dionex BioLC AD25 Absorbance Detector with DX-LAN is a precision dual-beam variable wavelength photometer designed for high-performance liquid chromatography (HPLC) and bioanalytical workflows. Featuring an integrated holmium oxide filter for wavelength verification, this detector supports continuous wavelength scanning from 190 nm to 800 nm with ±1 nm wavelength accuracy and <5 nm bandwidth, enabling sensitive and reliable UV-Vis absorbance detection.

Key technical specifications include:

• Decibel level: 52 dB, ensuring low-noise performance critical in molecular diagnostics and bioprocessing.
• Autozero capability: 3 AU with ±100 µAU accuracy to maintain baseline stability during long-term chromatography runs.
• Noise: ±10 µAU peak-to-peak at 254 nm with 2-second rise time (flowing deionized water), supporting high sensitivity in low-concentration analyte detection.
• Drift: <100 µAU/hour post warm-up to guarantee consistent signal quality over extended experiments.
• Discrete filter rise times adjustable between 0.1 to 10 seconds for optimized signal smoothing according to sample complexity.
• Analog output dynamically configurable from 0.001 to 3.0 AU, suitable for diverse detector signal integration requirements.
• Flow rate accuracy and precision are <1% and <0.2% respectively, essential for reproducible biomanufacturing chromatography processes.
• Robust operational capacity withstanding pressures up to 35 MPa (5000 psi) and low system delay volumes (<400 μL low delay, <800 μL standard delay) to reduce dispersion and maintain peak integrity.

This absorbance detector is widely adopted in biotechnology research, synthetic biology, molecular diagnostics, and biopharmaceutical production pipelines, where precise absorption measurement is fundamental for sample characterization and quality control. Its compatibility with the BioLC system components facilitates seamless integration into complex HPLC setups, essential in gene editing workflows and bioprocess monitoring.

The Dionex AD25's reliable wavelength verification, high sensitivity, and customizable output make it a critical instrument in analytical laboratories focusing on live-cell analysis, fluorescence absorption overlap correction, and quantification of biomolecules in complex mixtures.

For additional BioLC system components and customization options, direct inquiry is recommended. Relevant technical literature and application notes are available to support deployment in molecular biology and clinical diagnostics environments.