Thermo Scientific Vanquish Flex Quaternary UHPLC System with Variable Wavelength and Fluorescence Detectors

The Thermo Scientific Vanquish Flex Quaternary UHPLC System is a modular high-performance liquid chromatography platform configured with a quaternary pump, split-loop autosampler, thermostatted column compartment, variable wavelength UV/Vis detector, fluorescence detector, and Chromeleon software v.7.3.1. It is suitable for pharmaceutical analysis, bioanalytical quantification, impurity profiling, stability testing, biomarker analysis, and complex separation workflows in biotechnology and molecular diagnostics.

Included Configuration

• Vanquish Quaternary Pump F, p/n VF-P20-A
• Serial dual-piston operating principle
• Flow range: 0.001–8 mL/min, in 1 uL/min increments
• Pressure range: 2–103 MPa; above 5 mL/min, pressure range decreases to 80 MPa
• Four solvent lines with low-pressure gradient proportioning
• Static mixer volume: 350 uL
• Dwell volume: 679 uL
• Built-in four-channel solvent degassing
• Flow accuracy: ±0.1%
• Flow precision: <0.05% RSD or <0.01 min SD, whichever is greater
• Pulsation: typically <1.0% or <0.2 MPa, whichever is greater
• Proportioning accuracy: ±0.5% of full-scale
• Proportioning precision: <0.15% SD
• Fully automated compressibility compensation independent of mobile-phase composition

• Vanquish Split Sampler FT, p/n VF-A10-A
• Split-loop injection with 1 injection unit
• Pressure range: 2–103 MPa
• Injection volume range: 0.01–100 uL, with 0.01 uL minimum step
• Injection volume accuracy: typically ±0.5% for 10 uL water
• Injection volume precision: <0.25% area RSD for 1 uL caffeine in water; typically <0.5% area RSD for 0.5 uL
• Injection cycle time: down to 8 s, depending on separation conditions
• Minimum sample required: 2 uL at 1 uL injection volume
• UV carryover: <0.002% with caffeine; typically <0.0004%
• External needle wash with one solvent per injection unit, dip rinse, and continuous rinse
• Sample compartment temperature range: 4–40 °C
• Sample temperature accuracy: −2 °C/+4 °C
• Sample temperature stability: ±1 °C
• Capacity for 4 SBS-format rack or well-plate segments, plus 12 × 22.5 mm outer-diameter vials in the carousel
• Includes 3 sample racks, each with 54 positions for 12 mm outer-diameter vials up to 1.5 mL
• Barcode functions include empty-segment detection, rack or well-plate verification, and inventory management

• Vanquish Column Compartment H, p/n VH-C10-A
• Still-air and forced-air column chamber
• Temperature range: 5–120 °C in 0.1 °C increments
• Temperature stability: ±0.05 K
• Temperature accuracy: ±0.5 K up to 80 °C
• Heating performance: <15 min from 20–50 °C at ±1 K; typically 5 min from 25–40 °C
• Cooling performance: <15 min from 50–20 °C at ±1 K
• Capacity: 2 column slots

Variable Wavelength UV/Vis Detector

The Vanquish Variable Wavelength Detector F, p/n VF-D40-A, uses a UV/Vis/NIR photometer with a tunable Czerny-Turner monochromator and an internal reference beam.

• Light sources: deuterium lamp for UV and visible wavelengths; tungsten lamp for visible and near-infrared wavelengths
• Wavelength range: 190–900 nm
• Spectral bandwidth: typically 6 nm at 254 nm
• Wavelength accuracy: ±1 nm
• Wavelength repeatability: ±0.1 nm
• Internal wavelength calibration using the zero order of the monochromator spectrum
• Internal wavelength validation using a holmium-oxide filter
• Up to 4 signal channels
• Data collection rate: up to 250 Hz in single-channel mode and up to 5 Hz in multi-channel mode
• Single-channel noise: <±2.5 uAU at 254 nm
• Multi-channel noise: <±10 uAU, typically <±7 uAU, at 254 nm and 280 nm
• Drift: <0.1 mAU/h at 254 nm
• Linearity: <5% at 2.5 AU, typically <3% at 2.5 AU
• Includes Standard Bio flow cell, p/n 6077.0200: 11 uL, 10 mm path length, 50 bar

Fluorescence Detector

The Vanquish Fluorescence Detector F, p/n VF-D50-A, provides selective fluorescence analysis using two monochromators with concave holographic gratings and elliptic mirrors.

• Light source: 15 W xenon flash lamp
• Photomultipliers: 1
• Excitation wavelength range: 220–630 nm
• Emission wavelength range: 220–650 nm
• Excitation and emission spectral bandwidth: 20 nm
• Wavelength accuracy: ±2 nm
• Wavelength repeatability: ±0.2 nm
• Scan modes: excitation, emission, and synchronous single-spectrum scans
• Up to 4 channels
• Data collection rate: up to 200 Hz
• Wavelength switching time: <250 ms
• Variable emission filter with 5 positions
• Includes Standard Bio fused-silica flow cell, p/n 6079.4230: 8 uL, 20 bar (2 MPa)

Applications

This pre-owned Thermo Scientific UHPLC system is configured for:

• Pharmaceutical assay and impurity profiling
• Trace detection of fluorescent biomarkers
• Protein and peptide bioanalysis
• Stability and degradation studies
• Multi-component mixture separation and identification
• Fluorescence analysis in biotechnology and life-science research
• Quantitative UV/Vis methods for pharmaceutical and molecular biology workflows

The system includes a PC loaded with Chromeleon software v.7.3.1, a solvent rack, intercommunication cables, standard power cords, and the specified detector flow cells and sample racks. The listed instrument was manufactured in 2022–2023 and underwent initialization, self-test, software communication, pump, autosampler, column compartment, detector, leak-sensor, and test-injection checks.