BD FACSymphony X-50 Five-Laser Multiparameter Flow Cytometer with HTS Sampler & Supply System

The BD FACSymphony X-50 Flow Cytometer is a high-performance multiparameter cell analyzer equipped with five lasers and capable of detecting up to 28 fluorescent colors simultaneously through 30 configurable detector channels. This system integrates a High-Throughput Sampler (HTS) compatible with 96- and 384-well plates, enabling automated, plate-based sample acquisition essential for bioprocessing and molecular diagnostics applications.

Optical Configuration:

• Lasers: UV 355 nm (65 mW), Violet 405 nm (200 mW), Blue 488 nm (400 mW), Yellow-Green 532 nm (100 mW), Red 637 nm (100 mW)
• 28 fluorescence parameters with capacity up to 50 parameters upon upgrade
• Forward scatter (FSC) and side scatter (SSC) detectors optimized for particle sizing and granularity
• Precise filter sets tailored to each laser for optimal spectral resolution

Performance Specifications:

• Fluorescence resolution: CV <3.0% for PI-stained cells (Full G0/G1 peak)
• Acquisition throughput: up to 40,000 events/second with bead standards
• Fluidics system supports adjustable sample flow rates: LO (12 µL/min), MED (35 µL/min), HI (60 µL/min)
• Low sample carryover (<0.5% in HTS mode) ensures high data integrity for high-throughput experiments

Fluidics and Sample Handling:

• Robust FACSFlow Supply System maintains stable fluidic pressure and flow
• Multiple flow modes (RUN, STANDBY, PRIME) for flexible operation
• High sensitivity and resolution allow discrimination of rare cell populations, critical for advanced immunophenotyping and single-cell analysis

Software and Workflow Integration:

Includes a 1-year subscription to De Novo FCS Express 7 Analysis Software (RUO version) featuring:

• Compatibility with diverse flow cytometry data formats (FCS, BD FACSDiva, Beckman Coulter, Cytobank, etc.)
• Advanced gating, kinetic analysis, and statistical tools essential for molecular biology and gene editing workflows
• Real-time data visualization and customizable automated reporting
• Cloud-enabled collaboration for team-based biotechnological research

Applications:

• Complex multicolor immunophenotyping for clinical diagnostics and biotechnology research
• Rare cell detection and characterization
• Intracellular cytokine signaling and cell cycle studies
• High-throughput screening essential in synthetic biology and biopharmaceutical production pipelines

Quality Assurance and Service:

The instrument undergoes comprehensive service testing, including:

• Optical alignment and laser power verification at mW precision
• Fluidics system decontamination and pressure profiling
• Calibration with standardized bead sets ensuring reproducible coefficient of variation (CV) values
• Validation of detector sensitivity and noise levels to maintain signal fidelity
• Comes fully warranted with options for on-site or remote installation and training

Keywords:

multiparameter flow cytometer, cell analyzer flow cytometer, five-laser flow cytometer, high-throughput sampler, immunophenotyping instrument, molecular diagnostics tool, bioprocessing analytics, synthetic biology research, rare cell detection, fluorescence detection system, advanced flow cytometry software

This BD FACSymphony X-50 Flow Cytometer is a robust platform widely adopted in molecular biology and biotechnology laboratories, optimizing workflows for gene editing studies, cell phenotyping, and biopharmaceutical development.