BD FACSymphony A5 High-Parameter Flow Cytometer with 5 Lasers, 29 Colors, HTS Sampler & Supply System

The BD FACSymphony A5 Flow Cytometer is a high-performance, multiparametric cell analysis system optimized for advanced biomedical research and biotechnology applications such as deep immune profiling, molecular diagnostics, and synthetic biology workflows. Featuring an optical configuration of 5 lasers (Blue 488 nm, Red 628 nm, Violet 405 nm, UV 355 nm, Yellow-Green 561 nm) delivering up to 29 simultaneous fluorescence colors with 31 detectors, this flow cytometer supports complex panel designs with potential upgrades up to 50 parameters.

Key technical features include:

• Laser Specifications:
• Blue 488 nm, 200 mW
• Red 628 nm, 200 mW
• Violet 405 nm, 200 mW
• UV 355 nm, 60 mW
• Yellow-Green 561 nm, 150 mW
• Detection: Enhanced forward scatter (FSC) and side scatter (SSC) detectors optimized for particle sizing and granularity resolution, with fluorescence sensitivity designed for rare cell population identification and low-expression marker detection.
• High-Throughput Sampler (HTS): Enables automated sample acquisition from 96- and 384-well plates with less than 0.5% carryover in high-throughput mode, ideal for high-throughput screening and bioprocessing workflows.
• Fluidics & Supply System: Stable, adjustable flow rates (LO: 12 µL/min; MED: 35 µL/min; HI: 60 µL/min), with system pressure stability to support extended run times and reliable sample handling.
• Software Integration: Includes a complimentary 1-year subscription to De Novo FCS Express 7 Analysis software (RUO version) for sophisticated, customizable, and publication-ready data analysis and visualization supporting diverse file formats typical in clinical diagnostics and molecular biology research.
• Performance Metrics: Proven fluorescence resolution (CV < 3.0%), linear fluorescence response, and acquisition rates up to 40,000 events/sec, confirmed via comprehensive calibration and quality control tests using standardized beads.

This system is widely adopted in fields such as immunology, neuroscience, and biopharmaceutical production pipelines for applications including multiparametric immunophenotyping, fluorescence analysis, rare cell detection, and phenotypic signaling comparisons across large sample sets.

The FACSymphony A5 is equipped with robust optical filter sets and alignment capabilities to ensure signal specificity and fidelity over time, essential for sensitive applications in gene editing and molecular cloning workflows. Its fluidics and optics are routinely validated to minimize noise and enable high-dimensional data acquisition compatible with modern biotechnological research demands.

Benefits include:

• Enhanced multiplexing capacity with 29-color detection and upgrade potential
• Automated, plate-based high-throughput sampling for large-scale studies
• Reliable fluidics system ensuring sample integrity and flow stability
• Integrated data analysis software suited for complex biological data interpretation
• Support for research requiring detailed cellular characterization and longitudinal studies in molecular diagnostics and synthetic biology

This pre-owned unit undergoes thorough servicing, calibration, and cleaning procedures to meet stringent quality standards, with a warranty and after-sales technical support. It represents a cost-effective, sustainable choice for academic, clinical, and biopharma laboratories seeking cutting-edge flow cytometry capabilities with seamless integration into biomanufacturing and clinical research workflows.