BD Accuri C6 Plus Benchtop 2-Laser, 4-Color Flow Cytometer for Fluorescence Analysis

The BD Accuri C6 Plus Flow Cytometer is a compact, benchtop flow cytometry system designed for multiparameter fluorescence analysis and cell characterization. Equipped with two lasers (blue 488 nm and red 640 nm), it supports four fluorescence detection channels optimized for common fluorochromes such as FITC, PE, PerCP, and APC, enabling detailed analysis critical in molecular diagnostics, cell biology, and biotechnology research.

Key technical features include:

• Laser configuration: 2-laser system (blue and red)
• Fluorescence channels: Four detectors with optical filters FL1 (533/30 nm), FL2 (585/40 nm), FL3 (670 nm LP), FL4 (675/25 nm) optimized for FITC, PE, PerCP, APC, and related dyes
• Flow rates: Variable from 10 to 100 µL/min with core sizes from 5 to 40 µm, tailored for small-volume samples
• Detection sensitivity: Minimum particle size detection down to 0.5 µm, suitable for rare event analysis and low-abundance populations
• Flow cell: 200 µm ID quartz capillary ensuring high resolution in scatter and fluorescence signals
• Data acquisition: Up to 10,000 events per second with 7.2 decades dynamic range, facilitating detailed data for complex gating strategies
• Fluorescence precision and linearity: <3% CV and ±0.05% linearity using chicken erythrocyte nuclei (CEN) standards
• Sample volume: Minimum 50 µL, optimized for precious or limited biological samples

Maintenance and support details:

• Preventative maintenance completed including fluidics cleaning, optical path inspection, and laser power validation
• New maintenance kit installed including fluid harness and pump tubing
• Delivered with a full set of bottles, rack, computer workstation, and 1-year subscription to FCS Express Analysis RUO version (with optional FlowJo subscription discount)
• Warranty: 120 days continental US; 60 days international with technical consultation and remote setup/training available

Applications suited for this system include:

• Apoptosis assays
• Intracellular calcium flux measurement
• Pluripotent stem cell phenotyping
• DNA content and ploidy analysis
• Marine and freshwater microbial community analysis
• Gene expression profiling

The BD Accuri C6 Plus is widely adopted in biotechnology fields such as gene editing workflows, synthetic biology experiments, and is critical in molecular cloning and bioprocessing pipelines. Its cost-efficiency, ease of maintenance, and high analytical sensitivity make it a favored instrument for both academic and industrial research labs focusing on clinical diagnostics, immunology, and biopharmaceutical production.

This system’s standardized fluorescence setup eliminates the need for detector voltage adjustments, providing consistent, reproducible results essential for rigorous experimental workflows. The peristaltic pump system offers stable flow conditions, vital for reproducible cytometric data.

In summary, the BD Accuri C6 Plus Flow Cytometer is an essential, pre-owned research instrument offering high-performance cellular analysis with reliable service history and comprehensive support, ideal for labs engaged in live-cell imaging, fluorescence analysis, and advanced biomedical engineering research projects.