Thermo Fisher Attune NxT Acoustic Focusing Cytometer V4B3Y4R3 with Autosampler, 4 Lasers, 14 Colors, 16 Detectors

The Thermo Fisher Attune NxT Acoustic Focusing Cytometer V4B3Y4R3 is an advanced benchtop flow cytometer optimized for multiparameter cell analysis and high-throughput applications in biotechnology and biomedical research. It employs acoustic focusing technology to precisely align cells, enhancing sensitivity, reducing clogging, and improving reproducibility critical for molecular diagnostics, immunophenotyping, and synthetic biology workflows.

Key technical features include:

• Laser and Detection Configuration: Four lasers (405 nm violet, 488 nm blue, 561 nm yellow, 637 nm red) enable excitation of up to 14 fluorescent colors detected by 16 photodetectors, facilitating comprehensive multi-color immunoassays and multiplexed protein expression analysis.
• Optical Filters: Specific bandpass filters are tuned for each laser channel to maximize fluorochrome signal fidelity and reduce spectral overlap, essential for detailed bioprocessing and gene editing research.
• Acoustic Focusing: Utilizes ultrasonic waves to align particles/cells within a flow stream, delivering high-precision particle sizing from 0.5 to 50 µm, supporting rare cell detection such as circulating tumor cells.
• Flow Rate and Volume: Adjustable flow rates from 12.5 to 1,000 µL/min accommodate sample analysis volumes ranging from 20 µL to 4 mL, allowing both limited and large sample throughput.
• Data Acquisition: Capable of capturing up to 35,000 events per second with 34 parameters, with a maximum electronic speed of 65,000 events/sec for comprehensive datasets in biopharmaceutical production pipelines and cellular phenotyping.
• Autosampler Compatibility: Supports 96-, 384-, and deep-well plates for automated high-throughput sample processing, reducing manual intervention and increasing throughput vital in biomanufacturing and molecular cloning workflows.
• No-Wash/No-Lyse Workflow: Enables rapid sample preparation minimizing assay complexity and potential artifacts, ideal for live-cell assays and clinical diagnostics.

The system includes a computer with proprietary Attune NxT software and a 1-year subscription to De Novo FCS Express 7 Analysis software (RUO), offering:

• Intuitive, user-friendly interface for complex data analysis and gating
• Advanced visualization (histograms, scatter plots, heat maps) for fluorescence data interpretation
• Automated, customizable reporting suitable for publication-ready outputs
• Support for multiple file formats, enhancing interoperability across diverse cytometry platforms

This cytometer is widely adopted in immunology, oncology research, neuroscience, and biotechnology fields where high-parameter flow cytometry and accurate multicolor analysis are essential. The instrument undergoes thorough quality control with standardized beads, alignment calibration, and fluidics decontamination ensuring precise and reliable performance. Optional extended fluid systems and sampling accessories are available to customize for specialized research needs.

Ideal applications encompass:

• Immunophenotyping and characterization of immune cell subsets for vaccine development
• Rare cell population detection in cancer and stem cell research
• Multiplex fluorescent assays in drug discovery and molecular biology
• Live-cell analysis and bioprocess monitoring in synthetic biology and gene editing

Thermo Fisher’s Attune NxT Acoustic Focusing Cytometer is a robust, flexible platform that supports diverse high-throughput, high-parameter flow cytometry workflows critical to cutting-edge biotechnology research, molecular diagnostics, and biopharmaceutical development.

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Keywords incorporated: Acoustic focusing flow cytometer, multi-laser flow cytometer, high-parameter cytometry, immunophenotyping, molecular diagnostics, synthetic biology research, bioprocessing, gene editing workflows, biopharmaceutical production pipelines, multiplex protein expression analysis, live-cell assays, molecular cloning workflows, rare cell detection, high-throughput cytometry.