Thermo Scientific Invitrogen BIGFOOT Spectral Cell Sorter with 4 Lasers, 27 Detection Channels

The Thermo Scientific Invitrogen BIGFOOT Spectral Cell Sorter is a high-throughput spectral flow cytometry instrument designed for advanced cell sorting applications in biotechnology, clinical diagnostics, and molecular biology research. This *benchtop spectral cell sorter* features:

• Optical configuration:
• 4 excitation lasers at 405 nm (Violet), 488 nm (Blue), 561 nm (Yellow-green), and 640 nm (Far-red)
• 27 fluorescence channels (7 Violet, 7 Blue, 7 Yellow-green, 4 Far-red) plus 2 light scatter detectors (FSC and SSC)
• Simultaneous pulse measurement of peak, area, and width on all channels to enable precise multiparametric analysis
• Fluorescence sensitivity below 100 MESF for standard fluorochromes such as FITC, PE, and APC

• Fluidics and sample handling:
• 100 µm ceramic nozzle operating at 30 PSI allows sorting of delicate and heterogeneous samples
• Supports 1.5 mL, 5 mL, 15 mL sample tubes, with automated temperature control from 4 °C to 37 °C for input and output fluidics
• Automated sample agitation tailored to tube type maintains sample homogeneity

• Sorting capabilities:
• Event acquisition rate exceeding 100,000 events per second across 60 parameters ensures rapid data collection
• Sorting speeds above 70,000 events per second with all parameters active, supporting multi-way sorting (up to 4-way) into 96-well and 384-well plates and various tube formats (1.5 mL to 50 mL)
• Automated droplet generation and stream alignment enhance precision and reproducibility
• Advanced software (SQS) supports spectral unmixing and conventional sorting modes

• Physical and operational details:
• Dimensions: 99 × 99 × 170 cm (WxDxH), weight approximately 650 kg
• Power supply: 100–240 VAC, 50/60 Hz compatible for global laboratory settings
• Includes comprehensive QC calibration wizards ensuring consistent performance over time

This spectral cell sorter is widely adopted in biotechnology and biomedical engineering fields for applications such as immunophenotyping, rare cell isolation, stem cell sorting, and cancer research. Its ability to handle complex multicolor panels makes it essential for molecular diagnostics and synthetic biology workflows requiring precise cell subset discrimination and isolation.

The instrument package includes sample holders, sorting adapters for multiple tube sizes, microplate stages, and dedicated computer with software, ensuring complete functionality out of the box. Testing by expert service teams confirms all subsystems meet rigorous standards, providing reliable performance for high-throughput bioprocessing and biomanufacturing pipelines.

This pre-owned system is shipped fully serviced with warranty support and technical consultation available, making it a cost-effective solution for research institutions and biopharma companies seeking robust, scalable cell sorting solutions integral to gene editing and molecular cloning workflows.