Thermo Attune NxT Acoustic Focusing Flow Cytometer, 4 Lasers, 14 Colors, 16 Detectors with CytKick MAX Autosampler

The Thermo Attune NxT Acoustic Focusing Flow Cytometer is a pre-owned multiparameter flow cytometry system manufactured in 2023 and configured with 4 lasers, 14 colors, and 16 detectors. It is supplied with a Thermo CytKick MAX Autosampler for automated analysis of microplates and tube racks.

Optical and Acquisition Configuration

• Laser allocation: violet/6, blue/2, yellow/3, and red/3
• Forward scatter and side scatter: 488/10 blue laser
• 405 nm violet laser filters: 450/40, 525/50, 610/20, 660/20, 710/50, 780/60
• 488 nm blue laser filters: 530/30, 695/40
• 561 nm yellow laser filters: 585/16, 620/15, 780/60
• 637 nm red laser filters: 670/14, 720/30, 780/60
• Flow rate: 12.5 to 1,000 µL/min
• Sample analysis volume: 20 µL to 4 mL
• Particle size range: 0.5 to 50 µm
• Data acquisition speed: up to 35,000 events/second with 34 parameters
• Maximum electronic speed: 65,000 events/second with all parameters

Acoustic focusing supports consistent sample delivery and rapid multiparameter analysis for immunophenotyping, rare-cell detection, oncology research, vaccine development, molecular biology, and biotechnology workflows.

CytKick MAX Autosampler

The included CytKick MAX Autosampler, reference A38976, supports automated high-throughput flow cytometry using:

• Minimum sample volume: 50 µL; 70 µL at 1,000 µL/min
• Dead volume: 30 µL, except 50 µL at 500–1,000 µL/min
• Minimum sample acquisition volume: 20 µL
• Throughput: less than 45 minutes for a 96-well plate in High-Throughput mode
• Carryover: less than 0.5% in standard mode with two wash cycles; less than 1% in Boost Mode
• Passive cooling for 96-well U-bottom plates and microcentrifuge tube racks

Compatible formats include:

• 96 deep-well plates
• 96-well standard-depth plates
• 384 deep-well plates
• 384-well standard-depth plates
• 96-well and 384-well foil-covered plates
• 1.5 mL and 2 mL microcentrifuge tube racks, up to 24 tubes per rack

Included Equipment and Accessories

• Thermo Attune NxT Acoustic Focusing Cytometer, reference A29009
• Thermo CytKick MAX Autosampler, reference A38976
• Attune/CytKick foot alignment bracket
• Attune NxT focus, waste, wash, and shutdown bottles
• CytKick focus and waste bottles
• Attune NxT drip tray accessory
• CytKick MAX universal cooling adapter, P/N 100081492
• CytKick MAX extended cooling adapter, P/N 100063482
• CytKick MAX tube rack, P/N 100064241
• CytKick MAX tube cooling rack, P/N 100076823
• Computer with Attune Software v7.1.0 and software license dongle
• Monitor, keyboard, mouse, communication cables, and power cables
• One-year Research Use Only subscription to De Novo FCS Express 7 Analysis software

Software and Applications

The included FCS Express 7 software supports data import, gating, statistics, kinetic analysis, histograms, scatter plots, heat maps, automated reporting, and customizable data visualization. It is suitable for flow cytometry data analysis in immunology, cell biology, drug discovery, oncology, molecular diagnostics, gene expression studies, and biopharmaceutical research.

This system is well suited to long-tail applications such as high-throughput immunophenotyping, rare-cell population analysis, multiplexed protein expression studies, and automated flow cytometry workflows in biotechnology and biomedical research.