Thermo Attune NxT Acoustic Focusing Flow Cytometer, 3 Lasers, 11 Colors, 13 Detectors with Autosampler V4B3R3

The Thermo Attune NxT Acoustic Focusing Flow Cytometer is a pre-owned multicolor flow cytometer configured with 3 lasers, 11 colors, and 13 detectors, plus an Attune NxT Autosampler for automated sample loading and high-throughput plate workflows. Acoustic focusing aligns cells precisely within the flow stream, supporting sensitive, reproducible analysis with reduced clogging risk.

Optical Configuration

• Laser allocation: Violet/4, Blue/4, and Red/3
• Forward scatter: 488/10 blue laser
• Side scatter: 488/10 blue laser
• 405 nm violet laser: 440/50, 512/25, 603/48, and 710/50
• 488 nm blue laser: 530/30, 574/26, 695/40, and 780/60
• 637 nm red laser: 670/14, 720/30, and 780/60

Technical Specifications

• Flow rate: 12.5 to 1,000 µL/min
• Sample analysis volume: 20 µL to 4 mL
• Particle size range: 0.5 to 50 µm
• Data acquisition speed: Up to 35,000 events/second with 34 parameters
• Maximum electronic speed: 65,000 events/second with all parameters
• Sample preparation: No-wash, no-lyse workflow
• Tube cycle time: 20–40 minutes
• Autosampler compatibility: 96-well, 384-well, and deep-well plates
• 96-well plate cycle time: Less than 42 minutes
• 384-well plate cycle time: Less than 180 minutes

The integrated autosampler supports automated plate-based flow cytometry for immunophenotyping, cell cycle analysis, apoptosis detection, rare event detection, microbial analysis, and high-throughput screening. The system is suitable for biomedical research, biotechnology, molecular biology, cell analysis, and biopharmaceutical research workflows.

Included Equipment and Software

• Thermo Attune NxT Flow Cytometer
• Attune NxT Autosampler
• Computer with monitor, mouse, keyboard, Attune NxT Software, and license dongle
• De Novo FCS Express 7 Research Use Only analysis software subscription
• Communication and power cables

FCS Express supports flow cytometry data import, gating, statistics, kinetic analysis, visualization, automated reporting, and compatibility with formats including FCS, LMD, CSV, BD FACSDiva, Beckman Coulter, Accuri C6, SPADE, Cytobank, and TXT files.

Available Configuration Options

• Attune NxT Extended Fluid System EFS1
• Attune Cytkick Sampler
• Alternative fluorescent channel allocations
• Alternative color-channel filters

The instrument was tested by an in-house service team for startup and initialization, software communication, pumps, valves, fluidics priming, cleaning cycles, baseline performance, Attune Performance Tracking Beads, and autosampler operation. It is a practical pre-owned flow cytometry platform for high-parameter cell analysis and high-throughput biotechnology applications.