Thermo Attune NxT AFC2 Acoustic Focusing Flow Cytometer with CytKick Autosampler, 2 Lasers, 9 Colors, 11 Detectors

The Thermo Attune NxT AFC2 acoustic focusing flow cytometer is a pre-owned benchtop cell analyzer configured with 2 lasers, 9 fluorescent colors, and 11 detectors. Acoustic focusing aligns cells into a single-file stream for multicolor flow cytometry applications including immunophenotyping, cell viability and apoptosis analysis, cell cycle analysis, protein expression, rare cell detection, and high-throughput sample screening.

Instrument Configuration

• Laser configuration: 2 lasers
• 488 nm blue laser with 3 channels
• 405 nm violet laser with 6 channels
• Detector configuration: 11 detectors
• Forward scatter: 488/10 blue laser
• Side scatter: 488/10 blue laser
• 488 nm blue laser filters: 530/30, 574/26, 695/40
• 405 nm violet laser filters: 450/40, 525/50, 610/20, 660/20, 710/50, 780/60
• Flow rate: 12.5 to 1,000 µL/min
• Sample analysis volume: 20 µL to 4 mL
• Particle size range: 0.5 to 50 µm
• Data acquisition speed: up to 35,000 events/second with 34 parameters
• Maximum electronic speed: 65,000 events/second with all parameters
• Tube cycle time: 20–40 minutes
• Workflow: no-wash, no-lyse sample preparation

CytKick Autosampler

The included Thermo CytKick Autosampler supports automated plate-based flow cytometry for biotechnology, cell biology, and high-throughput research workflows.

• Minimum sample volume: 50 µL; 70 µL at 1,000 µL/min
• Dead volume: 30 µL, except 50 µL at 500–1,000 µL/min
• Minimum sample acquisition volume: 20 µL
• Throughput: less than 45 minutes for a 96-well plate in High-Throughput mode
• Carryover: less than 0.5% in standard mode using 2 wash cycles
• Compatible vessels:
• 96 deep-well plates
• 96-well standard-depth plates
• 384 deep-well plates
• 384-well standard-depth plates
• 1.5 mL and 2 mL microcentrifuge tubes
• 96-well foil-covered plates

Included Equipment and Software

• Thermo Attune NxT Flow Cytometer, year of manufacture 2024
• Thermo CytKick Autosampler, year of manufacture 2022
• Side module with waste and focusing fluid bottles
• Computer with monitor, mouse, keyboard, Attune Cytometric Software, and license dongle
• De Novo FCS Express 7 Analysis Software, 1-year Research Use Only subscription
• Communication and power cables

The included FCS Express 7 analysis software supports flow cytometry data import, gating, statistics, kinetic analysis, histograms, scatter plots, heat maps, automated reporting, and data visualization. Compatible file formats listed include FCS, LMD, CSV, BD FACSDiva, Beckman Coulter, Accuri C6, SPADE, Cytobank, and TXT files.

Condition and Testing

The instrument was made in 2024 and is described as barely used, serviced, in stock, and fully functional. Testing included startup and initialization, software communication, pump and valve operation, fluidics priming, cleaning cycles, baseline testing with Attune Performance Tracking Beads, and autosampler operation.

An Attune NxT Extended Fluid System EFS1 and other filter colors may be available separately. This system is intended for research use, including flow cytometry, immunology, molecular biology, biotechnology, and high-throughput cellular analysis.