Thermo Fisher Attune NxT Acoustic Focusing Cytometer V4B3Y4R3 with Autosampler (4 Lasers, 14 Colors, 16 Detectors)

The Thermo Fisher Attune NxT Acoustic Focusing Cytometer V4B3Y4R3 is an advanced benchtop flow cytometry system designed for high-throughput, multiparameter cellular analysis. Utilizing acoustic wave technology for precise cell alignment, it enhances sensitivity and sample throughput while reducing typical clogging issues, essential for molecular diagnostics, bioprocessing, and biotechnology research.

Key technical specifications and features include:

• Optics & Lasers: Equipped with 4 excitation lasers (405 nm violet, 488 nm blue, 561 nm yellow, 637 nm red) enabling detection of up to 14 fluorescent colors across 16 photodetectors, supporting complex multiplexed assays in immunophenotyping and synthetic biology workflows.
• Flow Rate & Sample Volume: Flow rates ranging from 12.5 to 1,000 µL/min with sample volumes between 20 µL to 4 mL, suitable for a variety of sample types including small particles (0.5 to 50 µm).
• Data Acquisition: Capable of acquiring up to 35,000 events/second with 34 parameters, facilitating rapid data collection critical in cell-based assays and rare event detection such as circulating tumor cell analysis.
• Sample Handling: Integrated autosampler compatible with 96-well, 384-well, and deep-well plates offers automated, high-throughput sample loading with rapid cycle times (<42 min for 96-well plates), boosting productivity in large-scale molecular biology and drug discovery projects.
• No-Wash/No-Lyse Workflow: Supports simplified sample preparation protocols to maintain cell integrity and viability, advantageous in live-cell imaging and biomanufacturing quality control.
• Software Integration: Includes a complimentary 1-year Research Use Only (RUO) subscription to De Novo FCS Express 7 Analysis Software, providing advanced data visualization, customizable reporting, and compatibility with multiple file formats to streamline cytometry data analysis in clinical diagnostics and synthetic biology applications.

Additional key points:

• Acoustic focusing enhances signal quality and reduces instrument clogging, improving reproducibility and calibration stability critical for molecular diagnostics and gene editing research.
• Extensive testing and calibration protocols ensure linear flow rates, stable fluidics, and consistent laser and detector performance.
• Optional extended fluidics systems and sampler accessories available to customize throughput and assay flexibility.
• Supported by warranty and expert technical support including remote installation, training, and servicing, meeting the needs of academic, clinical, and industrial biotechnology laboratories.

This instrument is widely adopted in biotechnology fields such as immunology research, gene expression profiling, molecular cloning workflows, biopharmaceutical production pipelines, and vaccine development. Its multiparameter, high-throughput capabilities make it a critical tool for researchers requiring sensitive and precise single-cell analysis.

By integrating cutting-edge acoustic focusing technology with robust hardware and software solutions, the Attune NxT offers reliable performance for diverse biosciences applications centered on cellular phenotyping, fluorescence analysis, and synthetic biology research workflows.