Thermo Fisher Attune NxT Acoustic Focusing Flow Cytometer with Cytkick Autosampler (4 Lasers, 14 Colors, 16 Detectors)

The Thermo Fisher Attune NxT Acoustic Focusing Cytometer V4B3Y4R3 is a benchtop flow cytometry system designed for high-parameter cellular analysis in diverse research fields including molecular biology, oncology, immunology, and biotechnology applications such as molecular diagnostics and bioprocessing workflows. Utilizing acoustic wave technology for precise cell alignment, this cytometer enhances sensitivity and reduces clogging, optimizing data quality and throughput.

Key technical features include:

• Four laser excitation sources: Violet (405 nm), Blue (488 nm), Yellow (561 nm), Red (637 nm) lasers
• Fourteen fluorescence detection channels distributed across sixteen photodetectors enable simultaneous multi-color analysis
• Laser-channel allocation: Violet/4, Blue/3, Yellow/4, Red/3 with detailed bandpass filters for optimized spectral resolution
• Flow rate range: 12.5 to 1000 µL/min, accommodating sample volumes from 20 µL to 4 mL
• High-throughput autosampler: Attune Cytkick NxT compatible with 96-, 384-, and deep-well plates, supporting rapid batch processing (<42 min for 96-well)
• Data acquisition speed: Up to 35,000 events/second with 34 parameters measured concurrently; maximum electronic event rate up to 65,000 events/second
• No-wash, no-lyse sample preparation workflow, critical for live-cell assays and synthetic biology research where minimal sample manipulation preserves functional states
• Particle size detection range: 0.5 to 50 µm, suitable for single cells, beads, and microparticles

This system is supplied with:

• Attune NxT Software and a license dongle for instrument control and data acquisition
• De Novo FCS Express 7 Analysis software (1-year Research Use Only subscription) enabling advanced data visualization, gating, and statistical analysis compatible with multiple file formats from various flow cytometers
• Power and communication cables, along with technical support options including remote installation, training, and extended warranty availability

Applications include:

• Immunophenotyping for vaccine development and immune monitoring
• Rare cell detection such as circulating tumor cells in cancer research
• Gene expression profiling and multiplexed protein analysis in molecular cloning and synthetic biology studies
• High-content biopharmaceutical production monitoring in biomanufacturing pipelines

The instrument undergoes rigorous in-house quality control: fluidics system decontamination, optical alignment, laser power calibration, and electronic noise assessment to ensure reproducible coefficient of variation (CV) performance with standardized beads. This guarantees reliable scatter data and fluorescence sensitivity critical for accurate molecular diagnostics and cell phenotyping workflows.

The Attune NxT Acoustic Focusing Cytometer is a versatile platform widely adopted in biotechnology and biomedical engineering, delivering robust performance and scalability for complex multiparameter flow cytometry assays essential in cutting-edge research and clinical diagnostics.

Primary Keywords: Acoustic focusing flow cytometer, multi-laser cytometer, high-parameter flow cytometry Long-tail Phrases: Essential for molecular cloning workflows, widely adopted in synthetic biology research, critical in biopharmaceutical production pipelines, used for multiplexed protein expression analysis, popular among molecular biology researchers, high-throughput single-cell analysis platform.