Thermo Fisher Attune NxT Acoustic Focusing Flow Cytometer with 3 Lasers, 11 Colors, 13 Detectors and Autosampler

The Thermo Fisher Attune NxT Acoustic Focusing Cytometer V4B3Y4 is a high-performance benchtop flow cytometer designed for *multiparameter flow cytometry applications* in biomedical, biotechnology, and molecular diagnostics research.

• Optical Configuration:
• Three lasers: Violet (405 nm), Blue (488 nm), Yellow (561 nm)
• Detection channels: 11 fluorescent colors captured across 13 detectors
• Laser-to-channel allocation optimizes spectral resolution for complex panels in immunophenotyping and cell analysis
• Acoustic Focusing Technology:
• Employs acoustic waves to precisely align cells/particles within the flow stream, enhancing sensitivity and reducing clogging
• Enables high sample throughput and reproducible particle sizing critical for cell cycle, apoptosis, and rare event detection
• Sample Handling & Throughput:
• Integrated autosampler compatible with 96-, 384-, and deep-well plates, facilitating high-throughput screening workflows
• Flow rate adjustable from 12.5 to 1,000 µL/min with sample volumes ranging 20 µL to 4 mL
• Cycle time: <42 min for 96-well plate; <180 min for 384-well plate
• Data Acquisition and Performance:
• High-speed data collection: up to 35,000 events/second (34 parameters), max 65,000 events/second for all parameters
• No-wash, no-lyse sample prep workflow supports streamlined molecular diagnostics and gene editing assays
• Forward and side scatter channels for size and granularity measurements enhance phenotypic profiling accuracy
• Software & Analysis:
• Bundled with a complimentary 1-year subscription to De Novo FCS Express 7 RUO analysis software
• Features advanced data visualization, gating, and automated reporting essential for synthetic biology and bioprocessing research
• Supports multiple file formats ensuring interoperability across diverse research platforms
• Versatility & Applications:
• Suitable for immunophenotyping, microbial analysis, cell cycle analysis, apoptosis detection, rare event quantification, and high-throughput screening
• Widely adopted in biotechnology fields including synthetic biology, molecular cloning workflows, and biopharmaceutical production pipelines
• Service & Warranty:
• Fully tested with calibrated optics, fluidics, and electronics ensuring reproducible performance and signal fidelity
• Comes with continental US 90-day warranty, optional extended warranties, and remote or on-site training available
• Additional Technical Notes:
• Particle size detection range: 0.5 to 50 µm
• Instrument includes computer with licensed Attune NxT software, monitor, and peripherals
• Compatible with Attune NxT Extended Fluid System EFS1 and Cytkick Sampler accessory options for extended operation

This cytometer's precise acoustic focusing, combined with its multiparameter detection capabilities and automated sample handling, makes it an indispensable tool in biotechnology and clinical diagnostics laboratories focused on *advanced flow cytometry applications*, including gene editing validation, molecular diagnostics workflows, and high-throughput screening in bioprocess environments. The accompanying FCS Express software enhances data analysis efficiency, making it widely favored among molecular biology and biomedical research professionals.