Thermo Fisher Attune NxT Acoustic Focusing Cytometer with 4 Lasers, 14 Colors, 16 Detectors & Cytkick Autosampler

The Thermo Fisher Attune NxT Acoustic Focusing Cytometer is a high-parameter benchtop flow cytometer engineered for advanced cell analysis and molecular diagnostics applications. Utilizing acoustic wave technology for precise cell alignment, it enhances sensitivity and reduces clogging, enabling reproducible and high-throughput sample processing in biotechnology, molecular biology, and clinical research.

Key technical features include:

• Four lasers (405 nm violet, 488 nm blue, 561 nm yellow, 637 nm red) enabling multiplexed detection across 14 fluorescence colors and 16 photodetectors for comprehensive immunophenotyping and multi-parametric analysis.
• Sample flow rates from 12.5 to 1,000 µL/min with particle size range from 0.5–50 µm, accommodating diverse biological samples including rare cell populations and circulating tumor cells.
• Data acquisition speed up to 35,000 events/second (34 parameters) and maximum electronic speed of 65,000 events/second ensuring rapid data collection for high-throughput studies.
• Cytkick Autosampler compatible with 96-, 384-, and deep-well plates, supporting no-wash, no-lyse protocols for streamlined bioprocessing and synthetic biology workflows.
• Comprehensive configuration includes lasers with specialized optical filters for precise channel allocation, enabling multiplexed protein expression and gene expression profiling commonly required in molecular diagnostics and biopharmaceutical research.

The system is bundled with De Novo FCS Express 7 RUO software (1-year subscription), offering:

• Advanced data analysis tools including gating, statistics, and kinetic analysis.
• Support for multiple file formats (FCS, BD FACSDiva, Beckman Coulter, SPADE, Cytobank).
• Automated reporting and customizable visualization (histograms, scatter plots, heat maps).
• Seamless data integration and collaboration for multi-user research environments.

This cytometer's acoustic focusing technology, combined with multi-laser, multi-color detection, and autosampler automation, makes it critical for cutting-edge applications such as:

• Immunophenotyping and vaccine development
• Gene editing validation and molecular cloning workflows
• Detection and characterization of rare cell populations in oncology
• Multiplexed biomarker analysis in drug discovery and biomanufacturing pipelines
• High-throughput live-cell analysis in neuroscience and cell imaging studies

The instrument undergoes rigorous in-house servicing including fluidics decontamination, optical alignment, performance calibration with particle standards, and electronic noise verification to ensure optimal functional integrity under warranty. Optional on-site installation and remote training are available to facilitate adoption in research and clinical laboratories.

This Thermo Fisher Attune NxT Cytometer represents a robust solution for researchers requiring sensitive, high-throughput flow cytometry with multiplexing capacity tailored for complex biological assays in biotechnology, molecular diagnostics, and biomedical engineering domains.