Miltenyi MACSQuant VYB Flow Cytometer, 3-Laser, 8-Color, 10-Detector

Overview

The Miltenyi MACSQuant VYB flow cytometer is a pre-owned multicolor flow cytometry system configured with three integrated lasers, eight simultaneous fluorescence colors, and ten detectors. It is designed for immunophenotyping, fluorescent protein analysis, cell viability and apoptosis assays, and bead-based cytokine or surface-marker analysis.

The included MiniSampler supports automated processing of multiple samples using single-tube or automated sample handling workflows. The system is suitable for routine assays, biotechnology research, molecular biology, cell analysis, and core laboratory applications.

Optical Configuration

• Lasers: 3
• Laser wavelengths: 405 nm Violet, 488 nm Blue, and 561 nm Yellow
• Simultaneous detection: 8 colors
• Laser/channel allocation: Violet/2, Blue/2, Yellow/4
• Forward scatter: 561 nm Yellow laser
• Side scatter: 561 nm Yellow laser
• 405 nm Violet laser:
• V1: 425/45 nm VioBlue, BFP
• V2: 525/50 nm VioGreen
• 488 nm Blue laser:
• B1: 525/50 nm FITC, GFP
• B2: 593–650 nm PI, Lss-mKate
• 561 nm Yellow laser:
• Y1: 586/15 nm PE
• Y2: 615/20 nm mCherry, PE-Vio 615
• Y3: 661/20 nm mKate, APC
• Y4: 740 LP PE-Vio 770, APC-Vio 770

Performance Specifications

• Sample flow rate: 25–100 µL/min
• Sheath fluid consumption: 2–10 mL/min
• Maximum event rate: Up to 15,000 events/second
• Fluorescence sensitivity:
• FITC: <200 MESF, CV <5%
• PE: <100 MESF, CV <5%
• APC: <150 MESF, CV <5%
• Absolute cell count accuracy: ±7%
• Absolute cell count reproducibility: CV <5%

Sample Handling and Included Hardware

• MiniSampler for automated multiple-sample processing
• Single-tube sample holder
• MACS Chill 5 Rack
• Bottle caps with fluid-level sensors
• Keyboard and mouse
• Standard power cable
• Communication and power cables
• Instrument reference: 130-096-116

Physical and Electrical Specifications

• Dimensions with MACS MiniSampler: 669 mm × 500 mm width × depth
• Height: 394–553 mm, adjustable touchscreen
• Weight with MiniSampler: 51.5 kg / 113.5 lb
• Power requirements: 100–240 VAC, 50/60 Hz

Software

A complimentary 1-year subscription to De Novo FCS Express 7 Analysis software is included in the Research Use Only (RUO) version. The software supports flow cytometry data import, gating, statistical analysis, kinetic analysis, visualization, and automated reporting. Supported file formats include FCS, LMD, CSV, BD FACSDiva, Beckman Coulter, Accuri C6, SPADE, Cytobank, and TXT files.

Testing and Applications

The instrument has undergone functional testing, fluidics cleaning, touchscreen calibration, fluid-level sensor testing, valve and pump verification, MiniSampler testing, laser stability checks, optical inspection, laser alignment verification, and PMTV calibration using MACSQuant calibration beads.

Typical applications include:

• Immunophenotyping of cell populations
• Fluorescent protein expression quantification
• Cell viability and apoptosis analysis
• Bead-based cytokine and surface-marker analysis
• Multicolor cell analysis in biotechnology and molecular biology workflows