Agilent NovoCyte Quanteon Multiparameter Flow Cytometer, 4 Lasers, 25 Colors, 27 Detectors with NovoSampler Q and Fluidics Station II

The Agilent NovoCyte Quanteon multiparameter flow cytometer is configured with 4 solid-state lasers, 25 colors, and 27 detectors for high-parameter fluorescence analysis. The system includes an Agilent NovoSampler Q automated sample loader and NovoCyte Fluidics Station II for reproducible sample handling and fluid management.

Optical Configuration

• Laser configuration: 405 nm violet, 488 nm blue, 561 nm yellow, and 637 nm red
• Laser allocation: Violet/8 channels, Blue/7 channels, Yellow/6 channels, Red/4 channels
• Laser power: 100 mW per laser
• Laser beam profile: 10 × 60 µm elliptical beam
• Solid-state lasers include onboard thermoelectric cooling for thermal stability and beam quality
• Forward scatter and side scatter: 488 nm blue laser
• Fluorescence detection: Silicon photomultipliers (SiPMs), with one photodetector per channel
• FSC sensitivity: 0.4 µm
• SSC sensitivity: 0.1 µm
• Fluorescence threshold sensitivity: FITC ≤40 MESF, PE ≤10 MESF, APC ≤10 MESF, Pacific Blue ≤30 MESF
• Fluorescence resolution: <3% CV for CEN

The optical filter configuration includes:

• 405 nm violet laser: 445/45 nm, 525/45 nm, 586/20 nm, 615/20 nm, 667/30 nm, 695/40 nm, 725/40 nm, 780/60 nm
• 488 nm blue laser: 525/45 nm, 586/20 nm, 615/20 nm, 667/30 nm, 695/40 nm, 725/40 nm, 780/60 nm
• 561 nm yellow laser: 586/20 nm, 615/20 nm, 667/30 nm, 695/40 nm, 725/40 nm, 780/60 nm
• 637 nm red laser: 667/30 nm, 695/40 nm, 725/40 nm, 780/60 nm

Fluidics and Sample Handling

• Sample acquisition rate: 100,000 events/second
• Sample delivery: Positive-displacement syringe pump
• Volumetric absolute count precision: <5%
• Sample flow rate: 5 to 120 µL/min, continuously adjustable
• Sheath flow rate: 6.5 mL/min
• Sample acquisition volume: 5 µL to 5 mL
• Carryover: <0.1%
• Manual sample formats: 12 × 75 mm tube and 1.5 mL Eppendorf tube
• NovoSampler Q compatibility: 40-tube rack for 12 × 75 mm tubes; 24-well, 48-well, 96-well flat-, U-, and V-bottom plates; and 384-well microplates
• Fluid level sensing: Active weight-sensor monitoring with automated warnings
• Fluid container capacity: 3 L sheath, 3 L waste, 500 mL cleaning, and 500 mL rinse

Included Hardware and Software

• Agilent NovoCyte Quanteon flow cytometer, serial number V8B7Y6R4
• Agilent NovoSampler Q
• Agilent NovoCyte Fluidics Station II
• Fluidics bottles, caps, fluid harness, and cables
• 40-tube rack for 12 mm × 75 mm tubes
• Desktop computer with NovoExpress software trial
• Communication and power cables
• One-year subscription to De Novo FCS Express 7 Research Use Only analysis software

The system is suited to immunophenotyping, high-parameter flow cytometry, cell viability and apoptosis assays, stem cell characterization, immune cell analysis, bead-based assays, and quality-control testing. It supports biotechnology and biomedical research workflows involving complex cell populations, rare-event detection, assay development, and multiparameter fluorescence analysis.

Condition and Verification

This pre-owned instrument was manufactured in April 2023 and reported fully functional. Testing included fluidics priming, cleaning cycles, software connectivity checks, autosampler verification, fluidics sensor testing, baseline multi-peak bead testing, QC bead testing, and preventive maintenance before shipment.