Eppendorf BioFlo 320 Dual Bioprocess Control System for Single-Use Bioreactors

The Eppendorf BioFlo 320 Dual Bioprocess Control System is a versatile and compact benchtop bioreactor controller designed for both microbial fermentation and cell culture bioprocessing applications. It supports single-use BioBlu vessels and autoclavable glass/autoclavable vessels with volumes ranging from 0.3 L up to 50 L, facilitating flexible research workflows from lab scale to pilot scale-up.

Key technical specifications include:

• Gas flow management with up to 4-gas mixing capability (Air, O2, N2, CO2), employing thermal mass flow controllers (TMFC) for precise sparging and overlay gas supply (flow ranges between 0.002 and 20 SLPM depending on configuration).
• Integrated touchscreen interface for real-time monitoring and control of critical bioprocess parameters such as pH, dissolved oxygen (DO), temperature via PT100 probes, redox, and CO2 sensors.
• Four internal peristaltic pumps (Watson-Marlow heads) with variable speed controls for feeding and media addition, plus support for up to two optional external pumps to customize nutrient supply or reagent addition.
• Compatibility with heated blankets, Peltier exhaust condensers, and vessel scale connectors enables tight control of cultivation environments and biomass measurements.
• Robust digital inputs include up to three level sensors (for liquid and foam detection) and optional optical pH sensors to enhance process fidelity.
• Operating temperature ranges suit both single-use vessels (up to 45–80 °C depending on vessel) and jacketed stainless steel vessels with external chiller support for temperature-sensitive bioprocesses.
• Compact dimensions (40.6 cm W x 40.6 cm D x 66.0 cm H) and a net weight of 32 kg allow deployment in standard laboratory spaces.
• Power requirements: 100–120 / 208–240 VAC adaptable.

Ideal for biotechnology research, molecular diagnostics, bioprocess development, and biopharmaceutical production pipelines, this control system is extensively used in synthetic biology, gene editing workflows, and scale-up/scale-down studies. Its modular design enables customization with different agitation impellers (Rushton, marine, pitched blade), sparge TMFC sensors calibrated for broader flow ranges, and additional sensors/accessories tailored to specific upstream bioprocessing needs.

The system undergoes comprehensive in-house testing and servicing, ensuring full functionality of pumps, sensors, motor controllers, heating elements, and gas controls. Warranty and after-sales technical support are offered, with options for remote setup and operator training. The BioFlo 320 dual controller is a trusted solution for controlled fermentation and cell culture processes in medical, biotechnology, and academic laboratories focused on bioprocess optimization and live-cell culture monitoring.

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