Qiagen QIAcuity Four Digital PCR System with Software and Barcode Scanner

The Qiagen QIAcuity Four Digital PCR System is an integrated, four-plate nanoplate digital PCR platform for precise absolute quantification in molecular biology, biotechnology, oncology research, and molecular diagnostics. It combines reaction partitioning, thermal cycling, and fluorescence imaging in a single instrument.

• Processes up to 4 Nanoplates in parallel
• Compatible with 8-, 24-, and 96-well Nanoplate formats
• Daily throughput of up to 768 samples using 96-well formats and up to 192 samples using 24-well formats
• Nanoplate-based partitioning with thousands of reaction partitions
• 5-plex multiplex fluorescence detection
• Detection channels: Green, Yellow, Orange, Red, and Crimson
• High-power white LED excitation with an average output of 4750 lumens
• CMOS camera with 6.3 MP image acquisition
• 1 thermocycler
• Process temperature range: 35–99 °C
• Ramp rate: approximately 3 °C
• Temperature accuracy: ±1 °C
• Temperature homogeneity: ±1 °C
• Time to result: approximately 2 hours for the first plate and 80 minutes for each following plate
• Power requirements: 100–240 VAC, 50/60 Hz, 1000 VA

The QIAcuity Four supports end-point digital PCR workflows requiring high sensitivity, broad quantification range, and minimal hands-on processing. Typical applications include:

• Rare mutation detection in oncology research
• Copy number variation analysis
• Gene expression quantification
• Pathogen detection and viral load measurement
• Molecular diagnostics and biotechnology research
• Gene editing, synthetic biology, and biopharmaceutical development workflows requiring absolute nucleic acid quantification

Included equipment and accessories:

• Qiagen QIAcuity Four Digital PCR System
• Laptop with QIAcuity Software Suite v2.5.0.1
• Power and communication cables
• Barcode scanner

The pre-owned instrument was tested for system startup and shutdown, software communication, plate carrier loading and ejection, plate location sensor LEDs, system self-test, and extended self-test.