Bio-Rad QX200 Digital Droplet Reader for Precise Fluorescence ddPCR Quantification

The Bio-Rad QX200 Digital Droplet Reader is a specialized fluorescence detection module integral to the QX200 droplet digital PCR (ddPCR) platform, designed for precise quantitative molecular diagnostics and biotechnological applications. It processes completed 96-well droplet PCR plates, detecting fluorescence using a multi-pixel photon counter under LED illumination, supporting dual-channel assays with FAM (EvaGreen) and HEX/VIC dyes for multiplex detection.

Key features include:

• High throughput processing of up to 96 samples or approximately 1.5 million droplets per run
• Linear dynamic range spanning five orders of magnitude, enabling robust quantification across broad concentration gradients
• ±10% precision ensures reliable and reproducible data accuracy essential for gene editing validation, synthetic biology experiments, and bioprocess monitoring
• Integration with QX Manager Software Standard Edition v2.4 facilitates concentration calculations based on Poisson statistics and efficient data management
• Compatibility with both EvaGreen and probe-based assays supports diverse applications in molecular cloning, biomanufacturing quality control, and clinical diagnostics
• Requires Bio-Rad Automated or Manual Droplet Generator for droplet partitioning, enabling streamlined workflows in advanced biotechnological research

The QX200 platform, widely adopted in molecular biology and synthetic biology research, serves as a critical instrument in bioprocessing pipelines, molecular diagnostics, and biopharmaceutical production requiring sensitive and precise nucleic acid quantification. Its design supports continuous operation with multiple readers and maintains established assay protocols and 96-well plate formats, ensuring seamless integration into existing laboratory infrastructures focused on live-cell analysis, fluorescence quantification, and gene expression studies.

Dimensions of 66.0 x 53.3 x 30.5 cm and a weight of 26 kg facilitate placement on laboratory benches, while electrical compatibility (100–240V, 50/60Hz, 1A) supports global laboratory environments.

This droplet digital PCR reader is essential for researchers and clinical professionals involved in molecular diagnostics, gene editing verification, and biomanufacturing quality assurance, offering precision fluorescence analysis critical for advancing biotechnology and biomedical engineering applications.