Axon Axopatch 200B Patch Clamp Amplifier with CV 203BU Headstage, Digidata 1550B Digitizer and pCLAMP 10

The Axon Axopatch 200B patch clamp amplifier system is configured for low-noise voltage-clamp and current-clamp electrophysiology. The system includes a CV 203BU single-channel headstage, Axon Digidata 1550B digitizer, data acquisition PC, and pCLAMP 10 software license dongle.

Amplifier configuration

• Axopatch 200B microelectrode amplifier
• CV 203BU single-channel headstage
• Voltage-clamp and current-clamp operation
• Compatible with low-capacitance pipette holders
• Ultra-low-noise performance for stable gigaseal recordings
• Fast capacitive and series-resistance compensation
• Integrated low-pass Bessel filter with selectable cutoff frequencies
• Scaled analog outputs for real-time monitoring of I_Mon, V_Mon, and Command signals
• Capacitive feedback technology
• Suitable for single-channel, whole-cell, and excised-patch configurations

Digitizer configuration

The Axon Digidata 1550B low-noise data acquisition system provides:

• USB 2.0 high-speed interface
• Sampling rate up to 500 kHz per channel
• 16-bit A/D and D/A conversion
• 8 analog input channels
• 4 analog output channels
• 16 digital I/O lines, including 8 inputs and 8 outputs
• Separate A/D and D/A converters to reduce crosstalk
• Enhanced shielding for minimized digitizer-induced noise
• Digital synchronization port for imaging, stimulation, and other external hardware

Software and computer

The included data acquisition PC is configured for electrophysiology workflows with pCLAMP 10 or later, including:

• Clampex acquisition software
• Clampfit analysis software
• AxoScope
• USB software license dongle
• Driver support for the Axopatch amplifier and Digidata digitizer
• Programmable voltage and current protocols
• Seal testing and series-resistance compensation
• Leak subtraction and stimulus waveform design
• Online and offline filtering
• Peak analysis and event detection

Applications

This patch clamp recording system is suitable for:

• Whole-cell voltage-clamp and current-clamp experiments
• Single-channel recording
• Synaptic current analysis
• Action potential detection
• Drug-response assays
• Ion channel research
• Membrane biophysics
• Neuronal and cardiac electrophysiology
• Cardiac myocyte studies
• Neuropharmacology
• Optogenetics with synchronized digital outputs
• Calcium imaging and other integrated optical experiments

The high-speed, low-noise configuration supports precise voltage control, current measurement, real-time stimulus-response recording, and complex multiphase protocols. It is particularly relevant to neuroscience, cellular physiology, biotechnology research, and molecular pharmacology workflows.

Included components

• Axopatch 200B main amplifier unit
• 1 × CV 203BU headstage
• Axon Digidata 1550B digitizer
• Headstage interface cable
• USB 2.0 digitizer-to-PC cable
• Power supply and signal breakout cables
• Data acquisition PC
• pCLAMP 10 license dongle

Optional accessories such as additional headstages, pipette holders with integrated Ag/AgCl electrodes, Faraday cages, vibration isolation tables, perfusion systems, stimulus isolators, cameras, light sources, temperature controllers, and in-line solution heaters are available separately.