Thermo Scientific Invitrogen BIGFOOT Spectral Cell Sorter with 4 Lasers and 27 Detectors

The Thermo Scientific Invitrogen BIGFOOT Spectral Cell Sorter is a high-performance laboratory instrument designed for advanced multicolor flow cytometry and cell sorting in biotechnology, clinical diagnostics, and biomedical research. Featuring 4 excitation lasers (405 nm violet, 488 nm blue, 561 nm yellow-green, 640 nm far red) and 27 fluorescence detection channels, this spectral cell sorter enables comprehensive fluorescence analysis and spectral unmixing essential for complex immunophenotyping panels, rare cell isolation, and stem cell sorting workflows.

Key specifications include:

• Spectral Optics: 25 fluorescence plus 2 scatter parameters, simultaneous pulse peak, area, and width measurements ensure high-resolution data acquisition across violet, blue, yellow-green, and far red spectra.
• Fluidics System: 100 μm ceramic nozzle operating at 30 PSI, compatible with 1.5 mL to 50 mL sample tubes; temperature-controlled input/output (4°C to 37°C) with automatic agitation tailored to tube types, supporting sensitive bioprocessing and cell viability maintenance.
• Sorting Performance: Capable of >100,000 events/second acquisition and >70,000 events/second sorting with all parameters enabled, the system supports multi-way sorting into tubes and 96- or 384-well plates, facilitating complex experimental designs in molecular diagnostics and biomanufacturing.
• Automation & Calibration: Integrated automated drop delay adjustment and stream alignment with advanced calibration wizards promote consistent sort purity and reproducibility crucial for gene editing and synthetic biology research.
• Dimensions & Operation: Robust benchtop footprint (99 x 99 x 170 cm), weighing 650 kg, powered by 100-240 VAC 50/60 Hz supply. Includes comprehensive software and hardware accessories for sorting alignment and sample handling.

This cell sorter is widely adopted in biotechnology, cell imaging, and clinical research fields, providing reliable, high-throughput spectral cell sorting capabilities critical for molecular cloning workflows, biopharmaceutical production pipelines, and biomedical engineering applications. Designed for durability and precision, the BIGFOOT supports real-time spectral unmixing and conventional sorting modes, enabling detailed phenotypic and functional cell analyses within complex heterogeneous samples.

Primary keywords: spectral cell sorter, high-speed cell sorting, multicolor flow cytometry, fluorescence-activated cell sorting, immunophenotyping, cell sorting system Long-tail keywords: essential for molecular cloning workflows, widely adopted in synthetic biology research, critical in biopharmaceutical production pipelines, high-throughput spectral cell sorting for biotechnology, automated multiway cell sorting into 96/384-well plates, fluorescence sensitivity <100 MESF for FITC/PE/APC

This equipment package includes the cell sorter, SQS software, multiple sample holders and sort output adapters, enabling immediate integration into laboratory bioprocessing and diagnostic workflows. Warranty and after-sales technical support ensure reliability and serviceability for research and industrial environments.