Agilent 5400 Fragment Analyzer Automation-Ready Unit 1

Agilent 5400 Fragment Analyzer

The Agilent 5400 Fragment Analyzer is an automation-ready capillary electrophoresis system for DNA and RNA fragment analysis, sizing, quantification, and quality assessment. It uses automated multiplexed capillary electrophoresis with fluorescence-based detection, a high-sensitivity CCD detector, and LED excitation.

The system is suitable for NGS library quality control, genomic DNA integrity analysis, total RNA and small RNA evaluation, PCR product verification, and mRNA vaccine characterization and quality control. Its automation-ready design supports high-throughput molecular biology, biotechnology, genomics, and biopharmaceutical workflows.

Key Specifications

• Analysis method: Automated multiplexed capillary electrophoresis
• Detection: Fluorescence-based detection with high-sensitivity CCD detector and LED excitation
• Capillary array compatibility: 96 capillary arrays
• Sample capacity: 1 × 96-well PCR sample plate
• Samples per run: Up to 96 samples
• Recommended sample input: 2 µL
• Minimum volume per well for injection: 20 µL
• Resolution: Up to 3 bp
• Separation resolution: ≤5%
• Sizing accuracy: ±10%
• Power requirements: 100–240 VAC, 50/60 Hz
• Dimensions (H × W × D): 86.4 × 101.6 × 61 cm (34 × 40 × 24 in)
• Weight: 39 kg (86 lb)

Compatible Labware

• Full-skirt 96-well PCR plates for sample and marker preparation
• 96-well deep-well plates for buffer and waste reservoirs
• PCR microplate holders for marker and sample plates
• Deep-well microplate holders

Included Equipment and Accessories

• Agilent 5400 Fragment Analyzer
• Three marker/sample PCR microplate holders
• Two deep-well microplate holders
• One 50 mL bottle
• Two 250 mL bottles
• One 500 mL waste bottle
• Computer with Fragment Analyzer controller software
• Communication and power cables

Condition and Testing

This pre-owned Agilent 5400 Fragment Analyzer is described as fully functional, serviced, in stock, and in good cosmetic condition. Testing includes:

• Power supply stability
• Software communication and data exchange
• Initialization and homing processes
• Pump operation and fluid movement
• Plate movement and positioning
• Fluid priming
• Pressurization from minimum to maximum levels
• High-voltage system operation from minimum to maximum levels

Automation Options

Automation arms and automated liquid handlers may be available as configuration options. Capillary arrays and other consumables are not included and are used for demonstration purposes only.