Sartorius Biostat B Twin Bioreactor System for Microbial Cultures with 1L Jacketed Autoclavable Glass Vessels

The Sartorius Biostat B Twin is a versatile laboratory bioreactor system designed for controlled microbial fermentation applications, featuring dual 1-liter jacketed autoclavable glass vessels for parallel batch or fed-batch cultivation. This system is widely adopted in biotechnology and molecular biology workflows for strain screening, media optimization, and small-scale bioprocess development.

Key technical specifications include:

• Dual Vessel System: Two independent 1 L jacketed glass vessels with double-wall design enable precise temperature regulation and sterilization compatibility.
• Advanced Process Control: Integrated control of temperature, pH, dissolved oxygen (DO), agitation speed, and aeration supports sophisticated microbial culture conditions essential in bioprocessing and synthetic biology.
• Agitation and Aeration: Direct-drive 200 W motor delivers agitation speeds from 20 to 2000 rpm using a three-blade segment impeller, optimizing oxygen transfer and mixing efficiency in microbial fermentations. Aeration is managed via ring sparger with precise gas flow control, utilizing two Thermal Mass Flow Controllers (±1% full scale accuracy) for air and oxygen supply up to 3 L/min each.
• Multi-Channel Peristaltic Pumps: Four built-in Watson Marlow 114 pumps facilitate automated addition of acid, base, and substrates for controlled pH adjustment and nutrient feeding, critical for fed-batch processes and molecular diagnostics research.
• Sensor and Monitoring Capabilities: Equipped with pH and optical DO sensors with high accuracy (pH sensor compatible with Mettler Toledo InPro series; DO with VisiFerm DO or InPro 6800), plus precise temperature sensor (Pt100, ±0.1 °C accuracy), vessel and feed weight scales for gravimetric monitoring during operation.
• Temperature Management: Open thermostat system with recirculation pump and automatic cooling water valve supports culture temperature range up to 80 °C, essential for biomanufacturing process development.
• Connectivity and Data Integration: Multiple RS-232 and M12 plug digital inputs support accessory integration (e.g., external peristaltic pumps, exhaust condensers, rocker units), enabling advanced process automation and monitoring.

This bioreactor system is essential for biotechnology researchers focusing on microbial fermentation process development, including strain characterization, media optimization, and aerobic bioprocess engineering. It is popular among molecular biology and synthetic biology laboratories requiring reproducible, scalable bioprocess control in small working volumes.

Additional options include alternative vessel sizes up to 10 L, different impeller designs, heated exhaust filters, external chillers, and customized pump configurations, ensuring adaptability across diverse bioprocess applications.

The Sartorius Biostat B Twin undergoes thorough in-house testing to validate startup, sensor calibrations, peristaltic pump performance, gas flow accuracy with NIST-traceable standards, and full functionality of temperature and agitation controls prior to shipment. This reliable bioreactor solution comes with warranty support and remote training to facilitate smooth integration into your biotechnology workflow.

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