ALLSHENG Nano-500B Microvolume Combi-Cuvette Fluorometer Spectrophotometer, 2023

The ALLSHENG Nano-500B microvolume fluorometer and spectrophotometer measures UV-Vis absorbance and fluorescence for nucleic acid quantification, molecular biology laboratories, and OD600 cell density measurements.

The Nano-500B uses microvolume and combi-cuvette measurement formats. Its fluorescence configuration uses 460 ± 20 nm excitation and 525 to 570 nm emission wavelengths.

The unit is offered as used. It is described as new equipment in its original packaging and has a 2023 manufacturing year.

The ALLSHENG Nano-500B combines microvolume UV-Vis spectrophotometry, cuvette measurements, and fluorescence analysis in one benchtop instrument. It is suited to nucleic acid quantification, dsDNA analysis, and OD600 measurements used in molecular biology laboratories.

Measurement and optical specifications

• Minimum sample size: 0.5 to 2 μL
• Recommended sample size: 2 μL
• Path lengths: 0.05 mm, 0.2 mm, and 1 mm
• UV-Vis wavelength range: 200 to 800 nm
• Wavelength accuracy: ±1 nm
• Spectral resolution: ≤3 nm, FWHM at Hg 253.7 nm
• Absorbance precision: 0.003 Abs at 1 mm path length
• Absorbance accuracy: ±1% at 7.332 Abs and 260 nm
• Absorbance range: 0.04 to 300 at 260 nm, 10 mm equivalent
• Detection concentration range: 2 to 15,000 ng/μL dsDNA
• Detection time: below 6 seconds

Fluorescence specifications

• Fluorescence excitation wavelength: 460 ± 20 nm
• Fluorescence emission wavelength: 525 to 570 nm
• Fluorescence light source: blue LED
• Fluorescence linearity: R² ≥0.995
• Fluorescence repeatability: ≤1.5%
• Fluorescence stability: ≤1.5%

The specification also lists a xenon flash lamp under light source and life.

OD600 specifications

• Absorbance range: 0 to 4.000 Abs
• Absorbance stability: ≤0.5% from 0 to below 3 Abs, and ≤2% from 3 to 4 Abs
• Absorbance repeatability: ≤0.5% from 0 to below 3 Abs, and ≤2% from 3 to 4 Abs
• Absorbance precision: ≤0.005 A from 0 to 2 Abs, ≤1% from 2 to 3 Abs, and ≤2% from 3 to 4 Abs

Physical and electrical specifications

• Voltage input: DC 24 V, 2 A
• Power consumption: 25 W
• Dimensions: 208 × 320 × 186 mm, W × D × H
• Weight: 3.6 kg

The unit is offered as used, is described as new equipment in its original packaging, and was manufactured in 2023.

What these specifications mean

The 0.5 to 2 μL minimum sample size supports measurements where sample volume is limited. The recommended 2 μL volume provides the specified operating reference for microvolume analysis.

The 200 to 800 nm wavelength range covers UV-Vis measurements, while the fluorescence configuration targets blue excitation and green to yellow emission. The dsDNA detection range of 2 to 15,000 ng/μL supports both low and high concentration nucleic acid samples.

A detection time below 6 seconds supports rapid absorbance or fluorescence readings. The instrument also provides OD600 measurements across an absorbance range of 0 to 4.000 Abs for cell density analysis.