Zeiss AXIO Imager.Z1 Motorized Upright Fluorescence and Brightfield Microscope System

The Zeiss AXIO Imager.Z1 Fluorescence Motorized Microscope is a versatile upright research microscope designed for advanced brightfield and epi-fluorescence imaging applications critical in molecular diagnostics, biotechnology, and synthetic biology research. This system integrates a motorized 6-position fluorescence filter cube turret and objective turret, allowing seamless switching between multiple fluorochromes such as DAPI, FITC, Texas Red, and Cy5, facilitating complex fluorescence analysis workflows.

Key features include:

• Microscopy Techniques: Brightfield and fluorescence microscopy with options for phase contrast, differential interference contrast (DIC), and polarization contrast modular add-ons.
• Optics and Illumination:
• EXFO X-Cite 120Q metal halide light source with integrated light guide and collimating adapter ensures stable, uniform fluorescence excitation optimized for high-sensitivity imaging.
• Motorized motorized 0.9 NA condenser for precise light regulation.
• HAL100 12V 100W halogen illumination for brightfield transmitted light applications.
• Motorization and Automation:
• Motorized 6-position objective turret fitted with Zeiss Fluar objectives (5x, 10x, 20x) enabling multi-magnification imaging up to 200x with optional higher magnification apochromatic objectives.
• Motorized Z-axis with automated parfocality adjustment and programmable focus recall improves repeatability in Z-stacking and live cell imaging.
• Motorized XY Prior stage with PRIOR III control box and custom joystick enhances precise and reproducible sample positioning, critical for multi-position time-lapse and stitching workflows.
• Motorized filter cube turret for rapid switching between fluorescence channels suited for multiplexed fluorescent assays widely used in gene editing and bioprocessing research.
• Imaging and Camera:
• Integrated BUC5F-830EC 8.3MP CMOS color camera supports high-resolution image acquisition for quantitative fluorescence and brightfield microscopy.
• Trinocular phototube with configurable camera vs. eyepiece light splitting ratios to optimize image capture or direct observation.
• Supports open-source Micro-Manager software for multi-dimensional image acquisition including multi-frame (time lapse), multi-slice (Z stacking), and multi-position (XY tiling, mosaic stitching).
• Filter Sets Included: Choice of three new fluorescence filter sets including DAPI, EGFP/FITC, TRITC/Cy3, Texas Red, Cy5, compatible with common fluorescence dyes and molecular probes essential for cell imaging in biomedical engineering and synthetic biology.
• Customization and Upgrade Options:
• Additional motorized components (e.g., condenser, advanced filter sets).
• Upgraded illumination sources such as LED or xenon arc lamps.
• High-end CCD, sCMOS, or EMCCD camera upgrades for high-sensitivity biophotonics imaging.
• Expanded objectives including Zeiss Apochromat lenses for superior color correction and resolution.
• Applications:
• Widely used for fluorescence microscopy workflows integral to molecular cloning, gene editing validations, neuroimaging studies, and biopharmaceutical production pipelines.
• Suitable for core research facilities, clinical diagnostics, quality control, live-cell imaging, and multiplex fluorescence analysis.
• Service and Warranty:
• Fully serviced with preventive maintenance including optical distortion and fluorescence intensity calibration.
• Includes 180-day USA warranty and 60-day international warranty.
• Remote training and technical consultation available with customized setup to match laboratory requirements.

This motorized fluorescence microscope platform provides reliable, automated, and high-precision imaging necessary for biotechnology researchers aiming to enhance throughput and reproducibility in fluorescence-based assays and bioprocessing workflows. The Zeiss AXIO Imager.Z1's modular design and upgrade paths ensure it adapts to evolving research demands across molecular biology, synthetic biology, and biomedical engineering domains.