Nikon Ti2-E PFS N-SIM Super-Resolution Inverted Motorized Microscope with Dual Fluorescence Illumination

The Nikon Ti2-E PFS N-SIM is an advanced super-resolution inverted microscope designed for high-precision biological and biotechnological imaging workflows including molecular diagnostics, live-cell imaging, and synthetic biology research. This motorized microscope integrates dual fluorescence illumination paths—structured illumination laser-based (Nikon LU-N3-SIM) and widefield LED (Lumencor Sola)—enabling flexible multi-channel fluorescence and brightfield imaging applications essential for bioprocessing and gene editing studies.

Key features include:

• Motorized components: 6-position objective turret with Plan Apo λ and Apo oil immersion objectives; dual motorized 6-position filter cube turrets; motorized emission filter wheel; motorized XYZ stage with piezo nanopositioning for fast Z-stack acquisition and high-speed tile scanning.
• Perfect Focus System (PFS) for continuous autofocus and drift correction during long-term live-cell and time-lapse experiments.
• High-sensitivity Hamamatsu ORCA-Flash4.0 monochrome sCMOS camera supporting high-resolution, low-noise image acquisition crucial for molecular biology and biopharmaceutical imaging pipelines.
• Dual illumination automation for rapid switching between laser-based structured illumination microscopy (SIM) and LED widefield fluorescence, facilitating high-contrast imaging in cell imaging and fluorescence analysis applications.
• Environmental enclosure for thermal and airflow stabilization improves imaging consistency during live-cell experiments.
• Comprehensive software control via Nikon NIS-Elements AR enables multidimensional acquisition (XYZ, time, multiple fluorescence channels), automated stage mapping, and hardware synchronization, supporting high-throughput screening and synthetic biology assay development.

The system includes multiple new fluorescence filter sets (DAPI, FITC/EGFP, TRITC/Cy3, Texas Red, and Cy5-compatible), allowing highly specific excitation and emission suited for a broad range of fluorochromes commonly employed in biotechnology research. The modular design provides extensive options for upgrades or customization, including phase contrast, DIC, and additional light sources, enabling adaptation to diverse research needs such as molecular cloning workflows, biomanufacturing quality control, and live-cell fluorescence imaging.

This microscope is widely adopted in advanced biotechnology and biomedical engineering laboratories due to its combination of motorized automation, super-resolution imaging readiness, and flexible fluorescence capabilities. It is ideal for applications requiring precise multi-position scanning, rapid channel switching, and reliable long-term focus stability critical in gene editing experiments and synthetic biology research pipelines.

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Primary Keywords: inverted fluorescence microscope, super-resolution microscope, motorized fluorescence microscope, structured illumination microscopy, sCMOS camera imaging Long-Tail Keywords: automated multi-position stage microscope for live-cell imaging, fluorescence microscopy system for molecular diagnostics, SIM-ready microscope for biotechnology research, motorized fluorescence filter turret for bioprocessing, high-resolution imaging for gene editing workflows, fluorescence imaging system with Perfect Focus System (PFS) Domain-Specific Terms: bioprocessing, molecular cloning workflows, synthetic biology research, biomanufacturing, fluorescence analysis, live-cell imaging, multi-channel fluorescence, fluorescence filter wheel, microscopy automation, piezo nanopositioning, structured illumination microscopy (SIM)