Nikon Eclipse Ti-U Inverted Fluorescence Phase Contrast Microscope with 6-Position Filter Cube Turret and Mechanical XY Stage

The Nikon Eclipse Ti-U is a versatile inverted fluorescence microscope optimized for advanced biological and materials science research, providing essential capabilities for fluorescence imaging, phase contrast microscopy, and brightfield observation. It features a mechanical 6-position fluorescence filter cube turret compatible with multiple new filter sets (including DAPI, FITC, Texas Red, and Cy5 options), enabling precise excitation/emission wavelength selection for diverse fluorochromes widely used in molecular diagnostics, biotechnology, and cellular imaging workflows.

Key technical specifications include:

• Microscope Base: Nikon Eclipse Ti-U inverted platform with binocular head and dual camera ports for flexible observation and imaging.
• Illumination Sources: Nikon 100W full spectrum HBO lamp for fluorescence excitation; Nikon 100W halogen lamp for brightfield illumination, both with dedicated power supplies.
• Objectives: CFI Plan Fluor PhL and Ph1 objectives (4x/0.13, 10x/0.30, 20x/0.45) suitable for live-cell imaging, genetic engineering studies, and high-resolution phase contrast observation of unstained specimens.
• Stage: Mechanical XY stage compatible with slides, Petri dishes, and microplates, enabling precise sample positioning necessary for bioprocessing and synthetic biology research.
• Camera & Software: Included Bestscope 8.3MP color CMOS camera integrates with Micromanager and ImageJ for image acquisition, annotation, calibration, and quantitative fluorescence analysis critical in molecular cloning workflows and biopharmaceutical production pipelines.
• Customizability: Supports addition of phase contrast, DIC, polarization modules, motorized stages/shutter, higher magnification objectives, and advanced camera systems (CCD, sCMOS, EMCCD), allowing tailored configurations for clinical diagnostics and biomedical engineering applications.
• Fluorescence Filters: Three included new filter cubes selected from a palette optimizing excitation/emission for dyes such as DAPI, EGFP/FITC, TRITC/Cy3, Texas Red, and Cy5, enabling multi-channel fluorescence imaging essential in gene editing and cellular tracer studies.

The inverted design facilitates observation of thick, live specimens in culture vessels, supporting live-cell fluorescence imaging and dynamic cellular process monitoring. The microscope is widely adopted in neuroscience, cell biology, and pharmacology research due to its manual precision and flexibility.

This pre-owned unit is fully serviced, with optical systems carefully inspected for distortion and fluorescence intensity verified at the specimen plane. A 180-day USA warranty (extendable to one year) and remote training on hardware and software are included, ensuring reliable operation and integration into existing research workflows.

In summary, the Nikon Eclipse Ti-U inverted fluorescence phase contrast microscope is a technically robust imaging platform essential for researchers requiring adaptable fluorescence and phase contrast microscopy solutions, particularly in biotechnology, molecular biology, and clinical research domains. Its capability to combine brightfield, fluorescence, and phase contrast modalities makes it an indispensable tool for in-depth cellular and molecular analysis.