Nikon Eclipse Ti-U Inverted Fluorescence Microscope with Manual Stage and Multi-Modal Imaging

The Nikon Eclipse Ti-U is a versatile _inverted fluorescence microscope_ designed for detailed cellular imaging and analysis in biotechnology, molecular diagnostics, and clinical research. It features a durable mechanical 6-position filter cube turret equipped with three brand-new fluorescence filter sets such as DAPI, FITC, Texas Red, or Cy3, enabling multi-channel fluorescence imaging critical in synthetic biology and gene editing workflows. The microscope utilizes a 100 Watt full spectrum HBO lamp and halogen light sources to support fluorescence and brightfield microscopy techniques respectively, making it suitable for diverse research including live-cell imaging and fluorescence analysis.

Key technical specifications include:

• Microscope Base: Nikon Eclipse Ti-U inverted base with dual camera ports and illumination arm
• Optics: Mechanical 6-position turret accommodating CFI Plan Fluor and Plan Apo VC objectives ranging from 4x to 20x magnification, supporting up to 1000x with upgraded eyepieces/objectives
• Illumination: Integrated 100 W HBO lamp for fluorescence and 100 W halogen light source for brightfield
• Filter Sets: Three included new sets; DAPI (EX 365/28 nm), EGFP/FITC (EX 475/30 nm), Texas Red (EX 560/20 nm), with options for Cy5 compatible with high-end fluorochromes commonly used in bioprocessing and molecular biology assays
• Camera: Bestscope 8.3 MP color CMOS camera with C-mount adapter supporting high-resolution image and live video capture, compatible with ImageJ and proprietary software for extensive image analysis, annotation, and measurement
• Stage and Focus: Mechanical XY stage and mechanical condenser facilitate precise sample positioning and illumination control, essential for robust biotechnology experiments
• Customization & Upgrades: Options for phase contrast, DIC, polarized light, motorized stage, shutter, and advanced sCMOS/EMCCD cameras tailored for high throughput biomanufacturing research and synthetic biology applications

The inverted design supports observation of live cell cultures in Petri dishes, culture flasks, and microplates, making it indispensable for cellular imaging and biomedical engineering applications. Its manual controls offer flexibility and precision for researchers requiring hands-on adjustment during fluorescence microscopy and brightfield observations.

This microscope is particularly well-suited for:

• Fluorescence microscopy critical in molecular diagnostics and gene editing studies
• Live-cell imaging for cell biology and pharmacological research
• Material science and biomedical engineering sample analysis
• Biotechnological workflows including bioprocessing and synthetic biology applications

Serviced and quality-checked with confirmed optical uniformity and fluorescence intensity calibration, it includes a 180-day USA warranty and remote installation training, making it a reliable pre-owned instrument for research institutions and biopharma companies.

Keywords: inverted fluorescence microscope, multi-channel fluorescence imaging, Nikon Eclipse Ti-U, live-cell imaging, molecular diagnostics microscope, biotechnology imaging, fluorescence filter sets, HBO fluorescence lamp, CFI Plan Apo objectives, bioprocessing microscopy, gene editing imaging, biomedical engineering microscopy.