Nikon Ni-U Upright Fluorescence Microscope with New Fluorescence Filters and Metal Halide Illumination

The Nikon Ni-U Upright Fluorescence Microscope is engineered for high-quality fluorescence and brightfield imaging, optimized for biological and biotechnological research including molecular diagnostics, cell imaging, and live-cell fluorescence analysis. This microscope integrates the following technical features:

• Microscope Base and Optical Components:
• Nikon Eclipse Ni-U upright microscope with a C-TE2 trinocular head and CFI 10x/22 eyepieces.
• Objectives include Plan Fluor (4x/0.13, 10x/0.30) and a high-performance Plan Apo VC 20x/0.75 objective designed for enhanced resolution and contrast critical in bioprocessing workflows.
• Mechanical C-CSR XY stage and C-H2L slide adapter enable precise specimen navigation.

• Fluorescence Illumination and Filter System:
• EXFO X-Cite 120 Metal Halide light source with light guide ensures stable and intense excitation suitable for imaging standard fluorochromes in synthetic biology and molecular cloning workflows.
• NI-FLEI fluorescence light path coupled with Ni-FLT6 six-position turret for rapid switching between fluorescence filter sets.
• Includes new fluorescence filter cubes for DAPI, FITC/EGFP, Texas Red, TRITC, and Cy5 with spectral configurations optimized for common fluorophores such as AlexaFluor dyes. Key for multiplex fluorescence imaging and advanced molecular diagnostics.
• Optional filter upgrades and light source enhancements (LED, Xenon) available to match specific fluorochrome panels.

• Brightfield Illumination:
• D-LH 100W halogen lamp allows high-quality brightfield imaging for stained tissue or cell preparations, complementing fluorescence modalities.

• Imaging and Software:
• Bestscope 8.3MP color CMOS camera supports high-resolution image capture crucial for documentation in biomanufacturing and biomedical engineering applications.
• Compatible with open-source Micro-Manager software for multi-dimensional image acquisition, time-lapse, and automation, facilitating sophisticated live-cell imaging studies.

• Mechanical and Ergonomic Features:
• Ergonomic tube and adjustable stage height improve operational comfort during prolonged microscopy sessions.
• Camera ports configured for simultaneous eyepiece and digital imaging (50/50 split).

• Customization and Upgrades:
• Supports addition of phase contrast, DIC, polarization modules, and motorized components (filter turrets, shutter, nosepiece) to support complex fluorescence and differential contrast imaging needs in advanced biotechnology labs.
• Magnification range customizable between 40x and 200x with higher magnification Apochromat objectives available.
• Adaptable to diverse sample formats including slides, Petri dishes, and microplates.

• Service and Warranty:
• Delivered fully serviced with preventive maintenance completed. Optical paths checked for distortion and fluorescence intensity calibrated at the specimen plane.
• Comes with 180-day USA warranty (60-day international) with an option to upgrade to a 1-year warranty.
• Includes remote training for microscope operation and software use, supporting user proficiency in fluorescence microscopy critical for research reproducibility.

This microscope is widely utilized in life sciences research areas such as neuroscience imaging, cell biology, synthetic biology, and molecular diagnostics, making it a versatile instrument for bioprocessing and biomedical research environments. Its combination of stable metal halide fluorescence illumination, advanced optics, and high-quality imaging capabilities make it a preferred choice for labs requiring reliable fluorescence and brightfield microscopy platforms.