Nikon Eclipse Ti-E Inverted LED Fluorescence Motorized DIC Microscope with Motorized XY Stage

The Nikon Eclipse Ti-E is a high-performance inverted fluorescence microscope engineered for advanced cell imaging, biotechnology, and molecular diagnostics applications. It features a robust motorized LED fluorescence light source system combined with advanced differential interference contrast (DIC) capabilities for enhanced visualization of transparent specimens without staining. This model integrates multiple motorized components, including a motorized XY stage, Z-axis autofocus, variable filter cube turret, and six-position motorized objective turret, enabling precise automation essential for multi-dimensional imaging workflows.

Key technical specifications include:

• Fluorescence illumination from a Lumencor LED light source with customizable filter sets (DAPI, FITC, Texas Red, Cy3, Cy5 compatible), supporting a broad spectrum of fluorochromes common in gene editing, live-cell imaging, and synthetic biology research.
• Motorized six-position filter cube turret and motorized six-position objective nosepiece for seamless switching between fluorochromes and objectives.
• Objective options include Plan Fluor and Plan Apo VC lenses (4x/0.13, 10x/0.30, 20x/0.75) delivering high-resolution imaging over a magnification range configured up to 1000x.
• Brightfield illumination is provided by a 12V 100W halogen lamp with DIC optical components (polarizer, prisms, analyzer) for enhanced contrast imaging crucial in pharmacological and biological research.
• Integrated motorized XY stage with joystick control and LEP MAC6000 controller supports automated multi-position acquisition, mosaicking, and time-lapse imaging, widely adopted in molecular biology and bioprocessing workflows.
• Imaging capabilities include open-source Micromanager software with ImageJ for multi-frame, multi-position, and Z-stack acquisition, supporting quantitative analysis and real-time live-cell observation in biotechnology applications.
• Comes equipped with an 8.3MP Bestscope CMOS color camera and Dell Windows 10 laptop for immediate image capture and processing.

The system is fully service-tested with preventive maintenance, ensuring high optical quality, field uniformity, and fluorescence intensity performance at the specimen plane. Modular customization options include adding phase contrast, polarization, high-end CCD/sCMOS cameras, advanced fluorescence light sources (Xenon, Metal Halide), and motorized condensers—facilitating tailored solutions for synthetic biology, molecular cloning workflows, and biopharmaceutical production pipelines.

This inverted fluorescence microscope is critical in research fields such as cell biology, neuroscience, biotechnology, molecular diagnostics, and biomedical engineering. Its motorized automation and multi-dimensional imaging capabilities make it widely used in live-cell imaging, fluorescence analysis, and high-resolution structural studies.

In summary, the Nikon Eclipse Ti-E offers a highly flexible platform optimized for fluorescence and DIC microscopy with automated precision control, ideal for academic and industrial laboratories demanding rigorous imaging performance in biotechnology research.

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