Nikon Eclipse TI-E PFS Inverted LED Fluorescence DIC Motorized Microscope with Motorized Stage

The Nikon Eclipse TI-E PFS is a highly versatile motorized inverted fluorescence microscope optimized for advanced live-cell imaging, biotechnology, and molecular diagnostics applications. Equipped with a Lumencor LED light source and a six-position motorized fluorescence filter turret, it supports multiple fluorescence channels, including DAPI, FITC, Texas Red, Cy3, and Cy5 filter sets, enabling multiplexed imaging of labeled biomolecules.

Key features include:

• Motorized components: automated Z-axis drive, motorized XY stage with joystick control, motorized objective nosepiece (6-position turret), and motorized filter cube turret, enhancing automation and reproducibility for time-lapse, Z-stacking, and multi-position imaging workflows.
• Perfect Focus System (PFS): infrared-based autofocus mechanism that maintains continuous focus during long-term live-cell imaging, minimizing phototoxicity and photobleaching, crucial for bioprocess monitoring, neuronal activity studies, and embryo imaging.
• Differential Interference Contrast (DIC) capability integrated with brightfield illumination, improving contrast in unlabeled samples for detailed morphological analysis in biomedical engineering and material science research.
• Optical path optimized to reduce field distortion and ensure uniform illumination across the specimen plane.
• Comes with a Bestscope 8.3MP color CMOS camera and imaging software (Micromanager with ImageJ) offering multi-dimensional image acquisition (time-lapse, Z-slicing, mosaic/stitching) and quantitative analysis essential for biotechnology and cell biology studies.
• Compatible with various accessories and customizations including phase contrast, polarization modules, and higher performance APO objectives, enabling tailored configurations for specific molecular biology, synthetic biology, or pharmacological research needs.
• Controlled via a Dell laptop with Windows 10, ensuring integration with modern computational pipelines for image processing and automation critical in biomanufacturing research.

This microscope supports diverse applications such as fluorescence microscopy, time-lapse studies, DIC imaging, and multi-dimensional fluorescence analysis. It enables researchers to conduct stable, high-resolution imaging for gene editing validation, live-cell tracking, and drug response monitoring. The extensive motorization and automation capabilities reduce manual interventions, enhancing throughput and data consistency in complex biotechnology workflows.

Overall, the Nikon Eclipse TI-E PFS inverted fluorescence microscope is an advanced platform widely adopted in biomedical engineering, cell imaging, and clinical diagnostics laboratories aiming for precise, automated fluorescence and DIC imaging in live and fixed samples.