Nikon Eclipse Ni-U Upright Fluorescence Phase Contrast Microscope with Motorized Stage and New Fluorescence Filters

The Nikon Eclipse Ni-U Upright Fluorescence Phase Contrast Microscope is a versatile research instrument designed for advanced fluorescence, brightfield, and phase contrast microscopy. It features a motorized XY stage with mechanical Z-axis control, supporting precise and repeatable sample positioning required in biomedical engineering and molecular diagnostics workflows. This system is widely adopted in synthetic biology research and essential for molecular cloning workflows requiring high-quality fluorescence imaging.

Key technical specifications include:

• Optical Configuration: Upright trinocular head (C-TT) with widefield optics optimized for multi-modal imaging including brightfield, phase contrast, and fluorescence. Supports flexible eyepiece/camera port split configurations (100% eyepiece to 100% camera).
• Objectives: Equipped with Nikon Plan Fluar phase contrast objectives (4x, 10x, 20x) compatible with phase rings Ph1, Ph2, and Ph3, ideal for cell and tissue morphology studies.
• Illumination:
• Fluorescence: External X-Cite 120PC-Q Metal Halide light source with a manual 6-position fluorescence filter cube turret supporting three brand-new filter sets from DAPI, FITC/EGFP, Texas Red, TRITC/Cy3, or Cy5. These filter sets cover a broad spectral range for fluorescence channels widely used in biotechnology applications such as gene editing assays and live-cell imaging.
• Brightfield: Halogen 12V100W lamp with a 7-channel manual condenser (NI-CUD) outfitted with phase contrast inserts.
• Imaging and Camera: Integrated BestScope 8.3MP color CMOS camera (BUC5F-830EC) supports live video capture, time-lapse, and z-stacking through open-source Micromanager software. Enables detailed quantitative analysis and fluorescence intensity measurements critical in molecular diagnostics and bioprocessing research.
• Motorized Features: Motorized XY stage controlled via MCX-2 eco controller with joystick for automated slide scanning and multi-channel image acquisition workflows.
• Software Capabilities: Supports multi-dimensional image acquisition, annotation, calibration, and quantitative fluorescence analysis. Compatible with advanced microscopy automation and image processing techniques.

The microscope can be customized further for enhanced fluorescence capabilities, including LED or Xenon light sources, additional filter cubes, higher magnification APO objectives, and advanced sCMOS or EMCCD cameras. Common upgrades support biotechnology research including synthetic biology, biomanufacturing, and molecular cloning workflows.

This system includes a comprehensive 180-day warranty (continental USA) with an option to extend to 1 year, remote training for microscope operation and software use, and technical consultation to adapt configurations to specific research needs. It is a preferred choice in clinical diagnostics and biomedical research facilities for reliable fluorescence and phase contrast imaging with precision sample handling.

Applications:

• Fluorescence imaging for common and custom fluorophores in cell biology and molecular diagnostics
• Phase contrast microscopy for unstained live cell and tissue morphology
• Automated slide scanning and time-lapse studies for bioprocessing and gene editing experiments
• Educational and general research microscopy
• Quantitative fluorescence analysis in biopharmaceutical production pipelines

The Nikon Eclipse Ni-U balances superior optics, advanced fluorescence capabilities, and motorized automation, making it critical in biotechnology-focused imaging applications and widely used in neuroimaging, synthetic biology, and clinical research fields.