SEMROCK FF403/497/574-Di01-25x36 Multi-Edge Dichroic Beamsplitter for Epi-Fluorescence Microscopy

The SEMROCK FF403/497/574-Di01-25x36 is a precision-engineered multi-edge dichroic beamsplitter optimized for standard epi-fluorescence microscopy and imaging systems. Designed to handle broadband light sources such as LEDs and metal halide lamps, it features multiple spectral transitions that enable efficient separation of excitation and emission wavelengths critical for fluorescence analysis and live-cell imaging workflows.

Key technical specifications include:

• Transmission bands with average transmission (Tavg) > 90% at 414–450 nm, 507.5–528 nm, and 584–645 nm
• Reflection bands with average reflection (Ravg) > 97.5% at 370–393 nm, 466–490 nm, and 546–565 nm
• Edge wavelengths precisely set at 403 nm, 497 nm, and 574 nm for selective wavelength filtering in multichannel fluorescence applications
• Angle of incidence maintained at 45° ± 1.5° for optimal optical performance in microscopy optical paths
• High optical durability, resistant to degradation under ≥ 6.0 W power exposure, suitable for sustained biophotonics experiments and molecular diagnostics
• Flatness and wavefront quality supporting minimal optical distortion, with reflected wavefront error much greater than 6λ P-V at 632.8 nm
• Compact dimensions (25.2 mm × 35.6 mm ± 0.1 mm) and ≥ 80% clear elliptical aperture ensure compatibility with standard microscope filter cubes and imaging systems

This dichroic beamsplitter is essential for advanced fluorescence imaging modalities, widely used in biotechnology research, including gene editing validation, synthetic biology workflows, and bioprocess monitoring. Its robust construction and spectral performance make it a critical optical component in molecular biology laboratories and clinical diagnostics platforms specializing in biopharmaceutical production pipelines and fluorescence-based assays.

By integrating the SEMROCK FF403/497/574-Di01-25x36, researchers and laboratory professionals achieve precise spectral control and high-throughput fluorescence separation necessary for accurate, reproducible results in cell imaging and fluorescence analysis.