PerSeptive Biosystems CytoFluor 4000 Fluorescence and Bioluminescence Microplate Reader with 5–45°C Temperature Control

The PerSeptive Biosystems CytoFluor 4000 is a precision fluorescence and bioluminescence microplate reader designed for *quantitative analysis of soluble and cell-associated fluorescence signals*. It features temperature control from 5°C to 45°C in 0.2°C increments, supporting robust environmental conditions essential for live-cell imaging and various bioprocessing assays. Utilizing the low-noise, high-gain Electron Tubes, Inc. 9781 Photomultiplier Tube (PMT) within a lightproof detection chamber, the instrument ensures sensitive and reliable fluorescence intensity detection critical in molecular diagnostics and synthetic biology workflows.

Key technical specifications include:

• Excitation wavelengths: 320–700 nm
• Emission wavelengths: 390–800 nm
• Compatibility: Microplate formats with 6, 48, 96, and 384 wells, optimizing throughput in biotechnology and cell-based assay research
• Light source: Durable tungsten-halogen lamp of 50 watts with approx. 3,000 hours lifespan, suitable for prolonged assay operations in biopharmaceutical production pipelines
• Scan speed: 60 seconds or less per plate, ideal for high-throughput screening and gene editing applications
• Reconfigurable optics: Adapted for solution and cell-based assay measurements, enhancing versatility in cell imaging and molecular biology research
• Mix time range: 1 to 99 seconds, facilitating assay homogenization and reagent interaction

This reader operates on standard voltages (120V/220V, 50Hz/60Hz) and weighs approximately 21.77 kg (48 lbs), making it suitable for reliable performance in laboratory environments focused on biomedical engineering and molecular diagnostics. The CytoFluor 4000 aligns with harmonized code 902750, reflecting its classification as a chemical analysis instrument used extensively for electrophoresis and fluorescence-based quantitation methods.

This equipment supports advanced applications such as live-cell fluorescence assays, fluorescence resonance energy transfer (FRET) experiments, and high-throughput screening typical in biotechnology research facilities, molecular cloning workflows, and biomanufacturing quality control processes. It is a critical tool for laboratories requiring precise temperature-regulated fluorescence detection systems widely adopted in synthetic biology and clinical diagnostics domains.