BioTek Synergy HT Multi-Mode Microplate Reader with Absorbance, Fluorescence, and Luminescence Detection

The BioTek Synergy HT Microplate Reader is a versatile multi-detection system designed for *absorbance* (UV/Vis), *fluorescence*, and *luminescence* measurements critical in molecular diagnostics, biotechnology, and bioprocessing research. Featuring a dual optic design with separate fluorescence and absorbance channels, it accommodates standard plate formats ranging from 6 to 384 wells and PCR tubes up to 1.25″ in height, making it essential for applications like gene editing workflows and biomanufacturing.

Key technical specifications include:

• Absorbance Detection: Monochromator wavelength range of 200–999 nm adjustable in 1 nm increments, 2 photodiode detectors, dynamic range of 0–4.0 OD with 0.001 OD resolution, ideal for quantitative UV/Vis assays and spectral scanning.
• Fluorescence Detection: Deep blocking filters provide excitation from 300–650 nm and emission from 300–700 nm, with a photomultiplier tube (PMT) detection system and sensitivity down to 5 pM fluorescein, suitable for fluorescence analysis in live-cell imaging and synthetic biology research.
• Luminescence Detection: Low-noise PMT detecting wavelengths 300–700 nm with high sensitivity (30 amol ATP typical), supporting molecular diagnostics and bioluminescence assays.
• Temperature Control: Precise incubation from 4°C above ambient to 50°C (±0.5°C at 37°C) allows uniform kinetic assays critical in enzyme kinetics and cell-based assays.
• Shaking Capability: Built-in low, medium, high, and variable shaking modes support sample mixing for bioprocessing experiments.
• Read Methods: Include kinetic, end point, spectral scanning, and well area scanning with rapid read speeds—96 wells in 31 seconds and 384 wells in 80 seconds—facilitating high-throughput screening in clinical diagnostics and synthetic biology workflows.
• Integration: BioStack-ready for automation compatibility, enhancing throughput in biopharmaceutical production pipelines.

The Synergy HT employs a tungsten halogen lamp for absorbance and a xenon flash lamp optimized for UV/Vis detection. Its robust filter system minimizes excitation-emission light leakage to ensure precise fluorescence measurements. The system is computer-controlled via powerful Gen5 software for streamlined data acquisition and analysis, aiding researchers in biotechnology, cell imaging, and molecular cloning workflows.

*Note: The time-resolved fluorescence and dispenser modules are not included with this model.* Overall, the BioTek Synergy HT is a high-performance, multi-mode microplate reader widely adopted in synthetic biology research, molecular diagnostics, and bioprocessing environments requiring reliable and sensitive detection across multiple assay formats.