Teledyne Isco CombiFlash NextGen 300+ Automated Flash Chromatography System with UV and ELSD Detection

The Teledyne Isco CombiFlash NextGen 300+ is an advanced automated flash chromatography system designed for efficient purification and separation of complex compounds in research and biotechnological applications. Equipped with a dual detection system, it includes a variable UV detector operating over a 200–400 nm range for standard chromatographic analysis and an Evaporative Light Scattering Detector (ELSD) to enable detection of non-UV-active compounds, essential for synthetic biology and molecular diagnostics workflows.

Key technical specifications:

• Flow rate range: 1 to 300 mL/min supporting flexible bioprocessing scale separations
• Maximum operating pressure: Up to 20 bar (300 psi) with user-definable limits for versatile method development
• Solvent system: Configurable binary gradient formation with 1-4 solvent inlets allowing automatic solvent selection and gradient accuracy within ±2% of full scale
• UV Detection: Variable wavelength UV absorbance from 200 to 400 nm for comprehensive compound profiling
• ELSD parameters: Gas inlet pressure 60–70 psig; spray chamber temperature 10–60 °C; drift tube temperature 30–90 °C (max 60 °C above ambient); flow rate minimum 5 mL/min, enabling sensitive detection of diverse biomolecules
• Automated fraction collection: Accurate fraction arm positioning and RFID-enabled rack and column detection to minimize cross-contamination and improve reproducibility in compound isolation
• User interface: Intuitive touchscreen with manual control options enhances flexibility for method optimization in biotechnology and pharmaceutical research settings
• Robust fluidics and sensing: Includes check valves, waste and source-level sensors, ensuring stable gradient performance and baseline stability critical for biomanufacturing pipelines

Applications include purification of synthetic organic molecules, isolation and screening of target biomolecules, and preparative separation of compounds in biotechnology, clinical diagnostics, and chemical research. Its ELSD detection facilitates analysis of compounds lacking chromophores, making it indispensable in synthetic biology and molecular cloning workflows.

This system is widely adopted in molecular biology and biomedical engineering labs for method development, compound screening, and bioprocessing applications. Included accessories are solvent intake and waste hoses, compatible collection racks, and power cable, supporting turnkey installation.

Comprehensive factory testing verifies pump calibration, gradient accuracy, detector sensitivity, and fraction collector functionality, ensuring reliable performance and minimal downtime. The system is backed by a 120-day US and 60-day international warranty with remote installation and training options available, making it a reliable investment for research institutions and biopharma production pipelines.

Optimize your laboratory workflows with the CombiFlash NextGen 300+, a robust chromatography platform essential for high-throughput purification and advanced detection of complex biomolecules in biotechnology and synthetic biology research.